6JC3
The Cryo-EM structure of nucleoprotein-RNA complex of Newcastle disease virus
Summary for 6JC3
| Entry DOI | 10.2210/pdb6jc3/pdb |
| EMDB information | 9793 |
| Descriptor | Nucleocapsid, polyU (2 entities in total) |
| Functional Keywords | self-capping helical structure complex, nuclear protein, nuclear protein-rna complex, nuclear protein/rna |
| Biological source | Avian avulavirus 1 More |
| Total number of polymer chains | 2 |
| Total formula weight | 45622.74 |
| Authors | Song, X.,Shan, H.,Zhu, Y.,Ding, W.,Ouyang, S.,Shen, Q.T.,Liu, Z.J. (deposition date: 2019-01-28, release date: 2019-08-07, Last modification date: 2024-03-27) |
| Primary citation | Song, X.,Shan, H.,Zhu, Y.,Hu, S.,Xue, L.,Chen, Y.,Ding, W.,Niu, T.,Gu, J.,Ouyang, S.,Shen, Q.T.,Liu, Z.J. Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome. Elife, 8:-, 2019 Cited by PubMed Abstract: Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 Å resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode. This tightly packed structure functions as a seed for the assembly of a nucleocapsid from both directions, facilitating the growth of double-headed filaments with two separate RNA strings inside. Disruption of this structure by mutations in its loop interface yielded a single-headed unfunctional filament. PubMed: 31290740DOI: 10.7554/eLife.45057 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.8 Å) |
Structure validation
Download full validation report
