6JBP
Structure of MP-4 from Mucuna pruriens at 2.22 Angstroms
Summary for 6JBP
| Entry DOI | 10.2210/pdb6jbp/pdb |
| Related | 5DSS |
| Descriptor | Kunitz-type trypsin inhibitor-like 2 protein (2 entities in total) |
| Functional Keywords | protease inhibitor, mucuna pruriens, plant protein |
| Biological source | Mucuna pruriens (Velvet bean) |
| Total number of polymer chains | 1 |
| Total formula weight | 20110.43 |
| Authors | Jain, A.,Shikhi, M.,Kumar, A.,Kumar, A.,Nair, D.T.,Salunke, D.M. (deposition date: 2019-01-26, release date: 2020-01-29, Last modification date: 2024-11-20) |
| Primary citation | Jain, A.,Kumar, A.,Shikhi, M.,Kumar, A.,Nair, D.T.,Salunke, D.M. The structure of MP-4 from Mucuna pruriens at 2.22 angstrom resolution. Acta Crystallogr.,Sect.F, 76:47-57, 2020 Cited by PubMed Abstract: The structure of the MP-4 protein was previously determined at a resolution of 2.8 Å. Owing to the unavailability of gene-sequence information at the time, the side-chain assignment was carried out on the basis of a partial sequence available through Edman degradation, sequence homology to orthologs and electron density. The structure of MP-4 has now been determined at a higher resolution (2.22 Å) in another space group and all of the structural inferences that were presented in the previous report of the structure were validated. In addition, the present data allowed an improved assignment of side chains and enabled further analysis of the MP-4 structure, and the accuracy of the assignment was confirmed by the recently available gene sequence. The study reinforces the traditional concept that conservative interpretations of relatively low-resolution structures remain correct even with the availability of high-resolution data. PubMed: 32039885DOI: 10.1107/S2053230X20000199 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.217 Å) |
Structure validation
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