6J55

Crystal structure of an iron superoxide dismutate (FeSOD) from a pathogenic Acanthamoeba castellanii

Summary for 6J55

• Entry DOI: 10.2210/pdb6j55/pdb
• Descriptor: Superoxide dismutase, FE (II) ION (3 entities in total)
• Functional Keywords: iron superoxide dismutase, acanthamoeba castellanii, oxidoreductase
• Biological source: Acanthamoeba castellanii (Amoeba)
• Total number of polymer chains: 12
• Total formula weight: 284115.97

Authors

Lee, K.H.,Dao, T.O.,Asaithambi, K.,Na, B.K. (deposition date: 2019-01-10, release date: 2019-07-17, Last modification date: 2024-03-20)

Primary citation

Dao, O.,Asaithambi, K.,Na, B.K.,Lee, K.H.
Crystal structure of an iron superoxide dismutase from the pathogenic amoeba Acanthamoeba castellanii.
Acta Crystallogr.,Sect.F, 75:480-488, 2019

PubMed Abstract: The iron superoxide dismutase found in the pathogenic amoeba Acanthamoeba castellanii (AcFeSOD) may play essential roles in the survival of the parasite, not only by protecting it from endogenous oxidative stress but also by detoxifying oxidative killing of the parasite by host immune effector cells. The AcFeSOD protein was expressed in a stable form using an Escherichia coli expression system and was crystallized by the microbatch and hanging-drop vapour-diffusion methods. The structure was determined to 2.33 Å resolution from a single AcFeSOD crystal. The crystal belonged to the hexagonal space group P6 and contained 12 molecules forming three tetramers in the asymmetric unit, with an iron ion bound in each molecule. Structural comparisons and sequence alignment of AcFeSOD with other FeSODs showed a well conserved overall fold and conserved active-site residues with subtle differences.

PubMed: 31282867
DOI: 10.1107/S2053230X19008112

Experimental method

X-RAY DIFFRACTION (2.33000486301 Å)