6J43

Proteinase K determined by PAL-XFEL

Summary for 6J43

• Entry DOI: 10.2210/pdb6j43/pdb
• Descriptor: Proteinase K, CALCIUM ION (3 entities in total)
• Functional Keywords: hydrolase, serine proteiase, xfel, room temperature
• Biological source: Parengyodontium album (Tritirachium album,Engyodontium album)
• Total number of polymer chains: 1
• Total formula weight: 29038.95

Authors

Lee, S.J.,Park, J. (deposition date: 2019-01-07, release date: 2020-01-15, Last modification date: 2024-11-20)

Primary citation

Lee, D.,Park, S.,Lee, K.,Kim, J.,Park, G.,Nam, K.H.,Baek, S.,Chung, W.K.,Lee, J.L.,Cho, Y.,Park, J.
Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography.
J.Appl.Crystallogr., 53:477-485, 2020

PubMed Abstract: Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 Å resolution.

PubMed: 32280322
DOI: 10.1107/S1600576720002423

Experimental method

X-RAY DIFFRACTION (1.85 Å)