6J07

Crystal structure of human TERB2 and TERB1

6J07 の概要

• エントリーDOI: 10.2210/pdb6j07/pdb
• 関連するPDBエントリー: 5XUP
• 分子名称: Telomere repeats-binding bouquet formation protein 2, Telomere repeats-binding bouquet formation protein 1, ZINC ION (3 entities in total)
• 機能のキーワード: telomere, meiosis, protein-protein complex, nuclear envelope attachment, dna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19148.17

構造登録者

Wang, Y.,Chen, Y.,Wu, J.,Huang, C.,Lei, M. (登録日: 2018-12-21, 公開日: 2019-02-27, 最終更新日: 2024-11-13)

主引用文献

Wang, Y.,Chen, Y.,Chen, J.,Wang, L.,Nie, L.,Long, J.,Chang, H.,Wu, J.,Huang, C.,Lei, M.
The meiotic TERB1-TERB2-MAJIN complex tethers telomeres to the nuclear envelope.
Nat Commun, 10:564-564, 2019

PubMed Abstract: During meiotic prophase I, telomeres attach to and move on the nuclear envelope (NE), regulating chromosome movement to promote homologous pairing. Meiosis-specific proteins TERB1, TERB2 and MAJIN play a key role in this process. Here, we report the crystal structures of human TERB1-TERB2 and TERB2-MAJIN subcomplexes. Specific disruption of the TERB1-TERB2 or the TERB2-MAJIN interaction in the mouse Terb2 gene abolishes the telomere attachment to the NE and causes aberrant homologous pairing and disordered synapsis. In addition, depletion of SUN1 also partially disrupts the telomere-NE connection. We propose that the telomere-TRF1-TERB1-TERB2-MAJIN-NE interaction network and the telomere-LINC complex connection are likely two separate but cooperative pathways to stably recruit telomeres to the NE in meiosis prophase I. Our work provides a molecular model of the connection between telomeres and the NE and reveals the correlation between aberrant synapsis and the defective telomere attachment to the NE.

PubMed: 30718482
DOI: 10.1038/s41467-019-08437-1

実験手法

X-RAY DIFFRACTION (3.298 Å)