6J07
Crystal structure of human TERB2 and TERB1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 126.900, 126.900, 90.429 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.959 - 3.298 |
R-factor | 0.291 |
Rwork | 0.288 |
R-free | 0.33690 |
Structure solution method | SAD |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
High resolution limit [Å] | 3.298 | 7.100 | 3.300 |
Rmerge | 0.076 | 0.042 | 0.843 |
Rmeas | 0.079 | 0.044 | 0.885 |
Rpim | 0.021 | 0.012 | 0.257 |
Number of reflections | 6870 | 769 | 638 |
<I/σ(I)> | 5.9 | ||
Completeness [%] | 99.6 | 99.5 | 96.1 |
Redundancy | 14.7 | 13.2 | 9.9 |
CC(1/2) | 0.999 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 9 | 291 | 0.1 M Bicine pH 9.0, 2% (v/v) 1,4-Dioxane, 4% (w/v) Polyethylene glycol 20000 |
1 | EVAPORATION | 9 | 291 | 0.1 M Bicine pH 9.0, 2% (v/v) 1,4-Dioxane, 4% (w/v) Polyethylene glycol 20000 |