Crystal structure of 5A ScFv in complex with YFV-17D sE in prefusion state

Summary for 6IW2

DescriptorEnvelope protein E, Heavy chain of monoclonal antibody 5A, Light chain of monoclonal antibody 5A (3 entities in total)
Functional Keywordsyellow fever virus, neutralizing monoclonal antibody, envelope protein, flavivirus, viral protein, viral protein-immune system complex, viral protein/immune system
Biological sourceYellow fever virus (strain 17D vaccine) (YFV)
Total number of polymer chains18
Total molecular weight410867.59
Lu, X.S.,Xiao, H.X.,Li, S.H.,Pang, X.F. (deposition date: 2018-12-04, release date: 2019-02-13)
Primary citation
Lu, X.,Xiao, H.,Li, S.,Pang, X.,Song, J.,Liu, S.,Cheng, H.,Li, Y.,Wang, X.,Huang, C.,Guo, T.,Ter Meulen, J.,Daffis, S.,Yan, J.,Dai, L.,Rao, Z.,Klenk, H.D.,Qi, J.,Shi, Y.,Gao, G.F.
Double Lock of a Human Neutralizing and Protective Monoclonal Antibody Targeting the Yellow Fever Virus Envelope.
Cell Rep, 26:438-446.e5, 2019
PubMed: 30625326 (PDB entries with the same primary citation)
DOI: 10.1016/j.celrep.2018.12.065
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2737 0.1% 4.1% 2.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6iw2
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Molmil generated image of 6iw2
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Molmil generated image of 6iw2
rotated about y axis by 90°