6IW2

Crystal structure of 5A ScFv in complex with YFV-17D sE in prefusion state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 1
Unit cell lengths	95.967, 99.244, 123.099
Unit cell angles	96.39, 94.90, 100.36

Refinement procedure

Resolution	48.695 - 2.900
R-factor	0.2392
Rwork	0.237
R-free	0.27200
RMSD bond length	0.008
RMSD bond angle	0.895
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	48.700
High resolution limit [Å]	2.900	2.900
Number of reflections	84055
<I/σ(I)>	7
Completeness [%]	98.1
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.2 M magnesium chloride, 0.1 M Tris pH 8.0, 20 % w/v PEG 6000