6IV8

the selenomethionine(SeMet)-derived Cas13d binary complex

Summary for 6IV8

DescriptorThe selenomethionine (SeMet)-labeled Cas13d, RNA (51-MER), RNA (53-MER), ... (5 entities in total)
Functional Keywordscrispr, cas13d, crrna, binary complex, rna binding protein-rna complex, rna binding protein/rna
Biological sourceuncultured Ruminococcus sp
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Total number of polymer chains4
Total molecular weight250691.09
Authors
Zhang, B.,Ye, Y.M.,Ye, W.W.,OuYang, S.Y. (deposition date: 2018-12-02, release date: 2019-06-19, Last modification date: 2019-06-26)
Primary citation
Zhang, B.,Ye, Y.,Ye, W.,Perculija, V.,Jiang, H.,Chen, Y.,Li, Y.,Chen, J.,Lin, J.,Wang, S.,Chen, Q.,Han, Y.S.,Ouyang, S.
Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d.
Nat Commun, 10:2544-2544, 2019
PubMed: 31186424 (PDB entries with the same primary citation)
DOI: 10.1038/s41467-019-10507-3
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.15 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.22214 0.1% 10.3% 5.5% 0.39MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution