6IPC

Non-native human ferritin 8-mer

Summary for 6IPC

• Entry DOI: 10.2210/pdb6ipc/pdb
• Descriptor: Ferritin heavy chain, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• Functional Keywords: ferritin, 8-mer, inner disulfide bond, oxidoreductase
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 16
• Total formula weight: 326020.94

Authors

Zang, J.C.,Chen, H.,Zhao, G. (deposition date: 2018-11-03, release date: 2019-03-13, Last modification date: 2024-11-13)

Primary citation

Zang, J.,Chen, H.,Zhang, X.,Zhang, C.,Guo, J.,Du, M.,Zhao, G.
Disulfide-mediated conversion of 8-mer bowl-like protein architecture into three different nanocages.
Nat Commun, 10:778-778, 2019

PubMed Abstract: Constructing different protein nanostructures with high-order discrete architectures by using one single building block remains a challenge. Here, we present a simple, effective disulfide-mediated approach to prepare a set of protein nanocages with different geometries from single building block. By genetically deleting an inherent intra-subunit disulfide bond, we can render the conversion of an 8-mer bowl-like protein architecture (NF-8) into a 24-mer ferritin-like nanocage in solution, while selective insertion of an inter-subunit disulfide bond into NF-8 triggers its conversion into a 16-mer lenticular nanocage. Deletion of the same intra-subunit disulfide bond and insertion of the inter-subunit disulfide bond results in the conversion of NF-8 into a 48-mer protein nanocage in solution. Thus, in the laboratory, simple mutation of one protein building block can generate three different protein nanocages in a manner that is highly reminiscent of natural pentamer building block originating from viral capsids that self-assemble into protein assemblies with different symmetries.

PubMed: 30770832
DOI: 10.1038/s41467-019-08788-9

Experimental method

X-RAY DIFFRACTION (4.443 Å)