6IO4

Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A

6IO4 の概要

• エントリーDOI: 10.2210/pdb6io4/pdb
• 分子名称: Glyceraldehyde-3-phosphate dehydrogenase A, SILVER ION (2 entities in total)
• 機能のキーワード: silver, dehydrogenase, cytosolic protein
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 566319.52

構造登録者

Wang, H.,Sun, H.,Wang, M. (登録日: 2018-10-29, 公開日: 2019-07-03, 最終更新日: 2023-11-22)

主引用文献

Wang, H.,Wang, M.,Yang, X.,Xu, X.,Hao, Q.,Yan, A.,Hu, M.,Lobinski, R.,Li, H.,Sun, H.
Antimicrobial silver targets glyceraldehyde-3-phosphate dehydrogenase in glycolysis ofE. coli.
Chem Sci, 10:7193-7199, 2019

PubMed Abstract: Silver has long been used as an antibacterial agent, yet its molecular targets remain largely unknown. Using a custom-designed coupling of gel electrophoresis with inductively coupled plasma mass spectrometry (GE-ICP-MS), we identified six silver-binding proteins in . The majority of the identified proteins are associated with the central carbon metabolism of . Among them, we unveil that GAPDH, an essential enzyme in glycolysis, serves as a vital target of Ag in for the first time. We demonstrate that silver inhibits the enzymatic function of GAPDH through targeting Cys149 in its catalytic site. The X-ray structure reveals that Ag coordinates to Cys149 and His176 with a quasi-linear geometry (S-Ag-N angle of 157°). And unexpectedly, two Ag ions coordinate to Cys288 in the non-catalytic site with weak argentophilic interaction (Ag···Ag distance of 2.9 Å). This is the first report on antimicrobial Ag targeting a key enzyme in the glycolytic pathway of . The findings expand our knowledge on the mode of action and bio-coordination chemistry of silver, particularly silver-targeting residues in proteins at the atomic level.

PubMed: 31588287
DOI: 10.1039/c9sc02032b

実験手法

X-RAY DIFFRACTION (3.1 Å)