6IGY

Crystal structure of Aspergillus niger chitinase B

Summary for 6IGY

• Entry DOI: 10.2210/pdb6igy/pdb
• Descriptor: Glycosyl hydrolases family 18 family protein (2 entities in total)
• Functional Keywords: ostrinia furnacalis, chitinase, three-dimensional structure, chitin metabolism, hydrolase, (glcnac)5
• Biological source: Aspergillus niger
• Total number of polymer chains: 1
• Total formula weight: 45293.32

Authors

Liu, T.,Zhou, Y.,Yang, Q. (deposition date: 2018-09-27, release date: 2019-10-09, Last modification date: 2023-11-22)

Primary citation

Liu, T.,Han, H.,Wang, D.,Guo, X.,Zhou, Y.,Fukamizo, T.,Yang, Q.
Potent Fungal Chitinase for the Bioconversion of Mycelial Waste.
J.Agric.Food Chem., 68:5384-5390, 2020

PubMed Abstract: mycelial waste is a good raw material for production of -acetyl-d-glucosamine (GlcNAc). In this study, ChiB, an chitinase which is upregulated during autolysis, was found to degrade mycelial waste with high efficiency. It could produce 1.45 mM (GlcNAc) in 8 h from raw mycelial waste, outperforming other chitinases, including bacterial ChiA, human Cht, and insect ChtI and Chi-h. The crystal structure of ChiB was determined, and residues Trp and Trp were found to be important for the activity of ChiB toward mycelial waste; mutation of either Trp or Trp into phenylalanine or alanine resulted in dramatically decreased activity. A recombinant strain of was constructed to extracellularly produce ChiB, and the culture supernatant was used to treat mycelial waste. This eco-friendly strategy could produce 3.7 mM of GlcNAc from 10 g of mycelial waste in 94 h with a yield of 71.3%.

PubMed: 32275147
DOI: 10.1021/acs.jafc.0c01342

Experimental method

X-RAY DIFFRACTION (1.948 Å)