6IGB

the structure of Pseudomonas aeruginosa Periplasmic gluconolactonase, PpgL

Summary for 6IGB

• Entry DOI: 10.2210/pdb6igb/pdb
• Descriptor: Periplasmic gluconolactonase, PpgL, SULFATE ION, ACETATE ION, ... (4 entities in total)
• Functional Keywords: 6-phosphogluconolactonase activity, hydrolase
• Biological source: Pseudomonas aeruginosa PAO1
• Total number of polymer chains: 2
• Total formula weight: 78655.10

Authors

Song, Y.J.,Shen, Y.L.,Wang, K.L.,Li, T.,Zhu, Y.B.,Li, C.C.,He, L.H.,Zhao, N.L.,Zhao, C.,Yang, J.,Huang, Q.,Mu, X.Y. (deposition date: 2018-09-25, release date: 2018-11-21, Last modification date: 2023-11-22)

Primary citation

Song, Y.J.,Wang, K.L.,Shen, Y.L.,Gao, J.,Li, T.,Zhu, Y.B.,Li, C.C.,He, L.H.,Zhou, Q.X.,Zhao, N.L.,Zhao, C.,Yang, J.,Huang, Q.,Mu, X.Y.,Li, H.,Dou, D.F.,Liu, C.,He, J.H.,Sun, B.,Bao, R.
Structural and Functional Insights into PpgL, a Metal-Independent beta-Propeller Gluconolactonase That Contributes toPseudomonas aeruginosaVirulence.
Infect.Immun., 87:-, 2019

PubMed Abstract: Biofilm formation is a critical determinant in the pathopoiesis of It could significantly increase bacterial resistance to drugs and host defense. Thus, inhibition of biofilm matrix production could be regarded as a promising attempt to prevent colonization of and the subsequent infection. PpgL, a periplasmic gluconolactonase, has been reported to be involved in quorum-sensing (QS) system regulation. However, the detailed function and catalysis mechanism remain elusive. Here, the crystal structure of PpgL is described in the current study, along with biochemical analysis, revealing that PpgL is a typical β-propeller enzyme with unique metal-independent lactone hydrolysis activity. Consequently, comparative analysis of seven-bladed propeller lactone-catalyzing enzymes and mutagenesis studies identify the critical sites which contribute to the diverse catalytic and substrate recognition functions. In addition, the reduced biofilm formation and attenuated invasion phenotype resulting from deletion of confirm the importance of PpgL in pathogenesis. These results suggest that PpgL is a potential target for developing new agents against the diseases caused by .

PubMed: 30642898
DOI: 10.1128/IAI.00847-18

Experimental method

X-RAY DIFFRACTION (1.651 Å)