6I84
Structure of transcribing RNA polymerase II-nucleosome complex
Summary for 6I84
| Entry DOI | 10.2210/pdb6i84/pdb |
| EMDB information | 4429 |
| Descriptor | Histone H3.2, DNA-directed RNA polymerase II subunit RPB4, DNA-directed RNA polymerases I, II, and III subunit RPABC1, ... (21 entities in total) |
| Functional Keywords | nucleosome, elongation, chromatin, rna polymerase ii, transcription |
| Biological source | Xenopus laevis (African clawed frog) More |
| Total number of polymer chains | 23 |
| Total formula weight | 732091.00 |
| Authors | Farnung, L.,Vos, S.M.,Cramer, P. (deposition date: 2018-11-19, release date: 2019-01-02, Last modification date: 2024-05-15) |
| Primary citation | Farnung, L.,Vos, S.M.,Cramer, P. Structure of transcribing RNA polymerase II-nucleosome complex. Nat Commun, 9:5432-5432, 2018 Cited by PubMed Abstract: Transcription of eukaryotic protein-coding genes requires passage of RNA polymerase II (Pol II) through nucleosomes, but it is unclear how this is achieved. Here we report the cryo-EM structure of transcribing Saccharomyces cerevisiae Pol II engaged with a downstream nucleosome core particle at an overall resolution of 4.4 Å. Pol II and the nucleosome are observed in a defined relative orientation that is not predicted. Pol II contacts both sides of the nucleosome dyad using its clamp head and lobe domains. Structural comparisons reveal that the elongation factors TFIIS, DSIF, NELF, SPT6, and PAF1 complex can be accommodated on the Pol II surface in the presence of the oriented nucleosome. Our results provide a starting point for analysing the mechanisms of chromatin transcription. PubMed: 30575770DOI: 10.1038/s41467-018-07870-y PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.4 Å) |
Structure validation
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