6HYR

THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Q193C+MMTS

6HYR の概要

• エントリーDOI: 10.2210/pdb6hyr/pdb
• 関連するPDBエントリー: 6HY5
• 分子名称: Proton-gated ion channel, DODECYL-BETA-D-MALTOSIDE (2 entities in total)
• 機能のキーワード: pentameric transmembrane channel, ion channel., membrane protein
• 由来する生物種: Gloeobacter violaceus PCC 7421
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 178826.35

構造登録者

Hu, H.D.,Delarue, M. (登録日: 2018-10-22, 公開日: 2018-12-19, 最終更新日: 2019-01-23)

主引用文献

Hu, H.,Ataka, K.,Menny, A.,Fourati, Z.,Sauguet, L.,Corringer, P.J.,Koehl, P.,Heberle, J.,Delarue, M.
Electrostatics, proton sensor, and networks governing the gating transition in GLIC, a proton-gated pentameric ion channel.
Proc. Natl. Acad. Sci. U.S.A., 115:E12172-E12181, 2018

PubMed Abstract: The pentameric ligand-gated ion channel (pLGIC) from (GLIC) has provided insightful structure-function views on the permeation process and the allosteric regulation of the pLGICs family. However, GLIC is activated by pH instead of a neurotransmitter and a clear picture for the gating transition driven by protons is still lacking. We used an electrostatics-based (finite difference Poisson-Boltzmann/Debye-Hückel) method to predict the acidities of all aspartic and glutamic residues in GLIC, both in its active and closed-channel states. Those residues with a predicted pK close to the experimental pH were individually replaced by alanine and the resulting variant receptors were titrated by ATR/FTIR spectroscopy. E35, located in front of loop F far away from the orthosteric site, appears as the key proton sensor with a measured individual pK at 5.8. In the GLIC open conformation, E35 is connected through a water-mediated hydrogen-bond network first to the highly conserved electrostatic triad R192-D122-D32 and then to Y197-Y119-K248, both located at the extracellular domain-transmembrane domain interface. The second triad controls a cluster of hydrophobic side chains from the M2-M3 loop that is remodeled during the gating transition. We solved 12 crystal structures of GLIC mutants, 6 of them being trapped in an agonist-bound but nonconductive conformation. Combined with previous data, this reveals two branches of a continuous network originating from E35 that reach, independently, the middle transmembrane region of two adjacent subunits. We conclude that GLIC's gating proceeds by making use of loop F, already known as an allosteric site in other pLGICs, instead of the classic orthosteric site.

PubMed: 30541892
DOI: 10.1073/pnas.1813378116

実験手法

X-RAY DIFFRACTION (3.5 Å)