6HQV

Pentafunctional AROM Complex from Chaetomium thermophilum

6HQV の概要

• エントリーDOI: 10.2210/pdb6hqv/pdb
• 分子名称: Pentafunctional AROM polypeptide, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ZINC ION, ... (7 entities in total)
• 機能のキーワード: multifunctional enzyme, shikimate pathway, aromatic amino acid biosynthesis, 3-dehydroquinate synthase, 3-dehydroquinate dehydratase, shikimate dehydrogenase, shikimate kinase, 3-phosphoshikimate 1-carboxyvinyltransferase, 5-enolpyruvylshikimate-3-phosphate synthase, biosynthetic protein
• 由来する生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 352942.15

構造登録者

Arora Verasto, H.,Hartmann, M.D. (登録日: 2018-09-25, 公開日: 2020-05-06, 最終更新日: 2024-11-13)

主引用文献

Arora Veraszto, H.,Logotheti, M.,Albrecht, R.,Leitner, A.,Zhu, H.,Hartmann, M.D.
Architecture and functional dynamics of the pentafunctional AROM complex.
Nat.Chem.Biol., 16:973-978, 2020

PubMed Abstract: The AROM complex is a multifunctional metabolic machine with ten enzymatic domains catalyzing the five central steps of the shikimate pathway in fungi and protists. We determined its crystal structure and catalytic behavior, and elucidated its conformational space using a combination of experimental and computational approaches. We derived this space in an elementary approach, exploiting an abundance of conformational information from its monofunctional homologs in the Protein Data Bank. It demonstrates how AROM is optimized for spatial compactness while allowing for unrestricted conformational transitions and a decoupled functioning of its individual enzymatic entities. With this architecture, AROM poses a tractable test case for the effects of active site proximity on the efficiency of both natural metabolic systems and biotechnological pathway optimization approaches. We show that a mere colocalization of enzymes is not sufficient to yield a detectable improvement of metabolic throughput.

PubMed: 32632294
DOI: 10.1038/s41589-020-0587-9

実験手法

X-RAY DIFFRACTION (3 Å)