6HLM
Variant G129D of NuoEF from Aquifex aeolicus bound to NAD+
Summary for 6HLM
| Entry DOI | 10.2210/pdb6hlm/pdb |
| Related | 6HL2 6HL3 6HL4 6HLA 6HLI 6HLJ |
| Descriptor | NADH-quinone oxidoreductase subunit E, NADH-quinone oxidoreductase subunit F, FE2/S2 (INORGANIC) CLUSTER, ... (9 entities in total) |
| Functional Keywords | complex i, nuoef, electron transfer, aquifex aeolicus, electron transport |
| Biological source | Aquifex aeolicus More |
| Total number of polymer chains | 4 |
| Total formula weight | 138045.89 |
| Authors | Gerhardt, S.,Friedrich, T.,Einsle, O.,Gnandt, E.,Schulte, M.,Fiegen, D. (deposition date: 2018-09-11, release date: 2019-06-26, Last modification date: 2024-05-01) |
| Primary citation | Schulte, M.,Frick, K.,Gnandt, E.,Jurkovic, S.,Burschel, S.,Labatzke, R.,Aierstock, K.,Fiegen, D.,Wohlwend, D.,Gerhardt, S.,Einsle, O.,Friedrich, T. A mechanism to prevent production of reactive oxygen species by Escherichia coli respiratory complex I. Nat Commun, 10:2551-2551, 2019 Cited by PubMed Abstract: Respiratory complex I plays a central role in cellular energy metabolism coupling NADH oxidation to proton translocation. In humans its dysfunction is associated with degenerative diseases. Here we report the structure of the electron input part of Aquifex aeolicus complex I at up to 1.8 Å resolution with bound substrates in the reduced and oxidized states. The redox states differ by the flip of a peptide bond close to the NADH binding site. The orientation of this peptide bond is determined by the reduction state of the nearby [Fe-S] cluster N1a. Fixation of the peptide bond by site-directed mutagenesis led to an inactivation of electron transfer and a decreased reactive oxygen species (ROS) production. We suggest the redox-gated peptide flip to represent a previously unrecognized molecular switch synchronizing NADH oxidation in response to the redox state of the complex as part of an intramolecular feed-back mechanism to prevent ROS production. PubMed: 31186428DOI: 10.1038/s41467-019-10429-0 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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