6HKW

Crystal structure of human SDS22

6HKW の概要

• エントリーDOI: 10.2210/pdb6hkw/pdb
• 分子名称: Protein phosphatase 1 regulatory subunit 7, SULFATE ION (3 entities in total)
• 機能のキーワード: lrr protein, signaling protein, pp1 regulator
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 211668.58

構造登録者

Heroes, E.,Choy, M.S.,Page, R.,Peti, W.,Ulens, C.,Van Meervelt, L.,Nys, M.,Bollen, M. (登録日: 2018-09-09, 公開日: 2019-01-30, 最終更新日: 2024-01-17)

主引用文献

Heroes, E.,Van der Hoeven, G.,Choy, M.S.,Garcia, J.D.P.,Ferreira, M.,Nys, M.,Derua, R.,Beullens, M.,Ulens, C.,Peti, W.,Van Meervelt, L.,Page, R.,Bollen, M.
Structure-Guided Exploration of SDS22 Interactions with Protein Phosphatase PP1 and the Splicing Factor BCLAF1.
Structure, 27:507-518.e5, 2019

PubMed Abstract: SDS22 is an ancient regulator of protein phosphatase-1 (PP1). Our crystal structure of SDS22 shows that its twelve leucine-rich repeats adopt a banana-shaped fold that is shielded from solvent by capping domains at its extremities. Subsequent modeling and biochemical studies revealed that the concave side of SDS22 likely interacts with PP1 helices α5 and α6, which are distal from the binding sites of many previously described PP1 interactors. Accordingly, we found that SDS22 acts as a "third" subunit of multiple PP1 holoenzymes. The crystal structure of SDS22 also revealed a large basic surface patch that enables binding of a phosphorylated form of splicing factor BCLAF1. Taken together, our data provide insights into the formation of PP1:SDS22 and the recruitment of additional interaction proteins, such as BCLAF1.

PubMed: 30661852
DOI: 10.1016/j.str.2018.12.002

実験手法

X-RAY DIFFRACTION (3.09 Å)