6HJ2

Crystal structure of hPXR in complex with dabrafenib

Summary for 6HJ2

• Entry DOI: 10.2210/pdb6hj2/pdb
• Descriptor: Nuclear receptor subfamily 1 group I member 2, Dabrafenib, ISOPROPYL ALCOHOL, ... (4 entities in total)
• Functional Keywords: pregnane x receptor anti-cancer drug endocrine disruptor nuclear receptor, nuclear protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 36773.45

Authors

Granell, M.,Delfosse, V.,Bourguet, W. (deposition date: 2018-08-31, release date: 2020-03-25, Last modification date: 2024-06-05)

Primary citation

Schneider, M.,Delfosse, V.,Gelin, M.,Grimaldi, M.,Granell, M.,Heriaud, L.,Pons, J.L.,Cohen Gonsaud, M.,Balaguer, P.,Bourguet, W.,Labesse, G.
Structure-Based and Knowledge-Informed Design of B-Raf Inhibitors Devoid of Deleterious PXR Binding.
J.Med.Chem., 65:1552-1566, 2022

PubMed Abstract: Dabrafenib is an anticancer drug currently used in the clinics, alone or in combination. However, dabrafenib was recently shown to potently activate the human nuclear receptor pregnane X receptor (PXR). PXR activation increases the clearance of various chemicals and drugs, including dabrafenib itself. It may also enhance cell proliferation and tumor aggressiveness. Therefore, there is a need for rational design of a potent protein kinase B-Raf inhibitor devoid of binding to the secondary target PXR and resisting rapid metabolism. By determining the crystal structure of dabrafenib bound to PXR and analyzing its mode of binding to both PXR and its primary target, B-Raf-V600E, we were able to derive new compounds with nanomolar activity against B-Raf and no detectable affinity for PXR. The crystal structure of B-Raf in complex with our lead compound revealed a subdomain swapping of the activation loop with potentially important functional implications for a prolonged inhibition of B-Raf-V600E.

PubMed: 34958586
DOI: 10.1021/acs.jmedchem.1c01354

Experimental method

X-RAY DIFFRACTION (2.28 Å)