6HIP
Structure of SPF45 UHM bound to HIV-1 Rev ULM
Summary for 6HIP
| Entry DOI | 10.2210/pdb6hip/pdb |
| Descriptor | Splicing factor 45, HIV-1 Rev (41-49), TRYPTOPHAN, ... (5 entities in total) |
| Functional Keywords | splicing factor, viral protein, splicing |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 3 |
| Total formula weight | 24898.71 |
| Authors | Pabis, M.,Corsini, L.,Sattler, M. (deposition date: 2018-08-30, release date: 2019-03-27, Last modification date: 2024-01-17) |
| Primary citation | Pabis, M.,Corsini, L.,Vincendeau, M.,Tripsianes, K.,Gibson, T.J.,Brack-Werner, R.,Sattler, M. Modulation of HIV-1 gene expression by binding of a ULM motif in the Rev protein to UHM-containing splicing factors. Nucleic Acids Res., 47:4859-4871, 2019 Cited by PubMed Abstract: The HIV-1 protein Rev is essential for virus replication and ensures the expression of partially spliced and unspliced transcripts. We identified a ULM (UHM ligand motif) motif in the Arginine-Rich Motif (ARM) of the Rev protein. ULMs (UHM ligand motif) mediate protein interactions during spliceosome assembly by binding to UHM (U2AF homology motifs) domains. Using NMR, biophysical methods and crystallography we show that the Rev ULM binds to the UHMs of U2AF65 and SPF45. The highly conserved Trp45 in the Rev ULM is crucial for UHM binding in vitro, for Rev co-precipitation with U2AF65 in human cells and for proper processing of HIV transcripts. Thus, Rev-ULM interactions with UHM splicing factors contribute to the regulation of HIV-1 transcript processing, also at the splicing level. The Rev ULM is an example of viral mimicry of host short linear motifs that enables the virus to interfere with the host molecular machinery. PubMed: 30892606DOI: 10.1093/nar/gkz185 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.2 Å) |
Structure validation
Download full validation report
