6HH9

Crystal structure of a two-domain esterase (CEX) active on acetylated mannans co-crystallized with mannopentaose

Summary for 6HH9

• Entry DOI: 10.2210/pdb6hh9/pdb
• Related: 6HFZ
• Descriptor: GDSL-like protein, beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose, beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose, ... (4 entities in total)
• Functional Keywords: esterase, carbohydrate, galactoglucomannan, deacetylation, biomass, gut flora, sugar binding protein
• Biological source: Roseburia intestinalis L1-82
• Total number of polymer chains: 4
• Total formula weight: 171784.97

Authors

Michalak, L.,La Rosa, S.L.,Rohr, A.K.,Aachmann, F.L.,Westereng, B. (deposition date: 2018-08-27, release date: 2019-09-11, Last modification date: 2024-01-17)

• Primary citation: Michalak, L.,La Rosa, S.L.,Leivers, S.,Lindstad, L.J.,Rohr, A.K.,Lillelund Aachmann, F.,Westereng, B.; A pair of esterases from a commensal gut bacterium remove acetylations from all positions on complex beta-mannans.; Proc.Natl.Acad.Sci.USA, 117:7122-7130, 2020; PubMed: 32170022; DOI: 10.1073/pnas.1915376117

Experimental method

X-RAY DIFFRACTION (2.4 Å)