6HC2

Crystal structure of NuMA/LGN hetero-hexamers

6HC2 の概要

• エントリーDOI: 10.2210/pdb6hc2/pdb
• 分子名称: G-protein-signaling modulator 2, Nuclear mitotic apparatus protein 1 (2 entities in total)
• 機能のキーワード: cell division asymmetric cell division spindle orientation force generator, cell cycle
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 582036.46

構造登録者

Pasqualato, S.,Culurgioni, S.,Foadi, J.,Alfieri, A.,Mapelli, M. (登録日: 2018-08-13, 公開日: 2019-05-29, 最終更新日: 2024-01-17)

主引用文献

Pirovano, L.,Culurgioni, S.,Carminati, M.,Alfieri, A.,Monzani, S.,Cecatiello, V.,Gaddoni, C.,Rizzelli, F.,Foadi, J.,Pasqualato, S.,Mapelli, M.
Hexameric NuMA:LGN structures promote multivalent interactions required for planar epithelial divisions.
Nat Commun, 10:2208-2208, 2019

PubMed Abstract: Cortical force generators connect epithelial polarity sites with astral microtubules, allowing dynein movement to orient the mitotic spindle as astral microtubules depolymerize. Complexes of the LGN and NuMA proteins, fundamental components of force generators, are recruited to the cortex by Gαi-subunits of heterotrimeric G-proteins. They associate with dynein/dynactin and activate the motor activity pulling on astral microtubules. The architecture of cortical force generators is unknown. Here we report the crystal structure of NuMA:LGN hetero-hexamers, and unveil their role in promoting the assembly of active cortical dynein/dynactin motors that are required in orchestrating oriented divisions in polarized cells. Our work elucidates the basis for the structural organization of essential spindle orientation motors.

PubMed: 31101817
DOI: 10.1038/s41467-019-09999-w

実験手法

X-RAY DIFFRACTION (4.31 Å)