6HAQ

AFGH61B WILD-TYPE COPPER LOADED

Summary for 6HAQ

• Entry DOI: 10.2210/pdb6haq/pdb
• Related: 6H1Z 6HA5
• Descriptor: Endoglucanase, putative, COPPER (II) ION, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: lytic polysaccharide monooxygenase, oxidoreductase
• Biological source: Neosartorya fumigata (Aspergillus fumigatus)
• Total number of polymer chains: 2
• Total formula weight: 48776.77

Authors

Lo Leggio, L.,Poulsen, J.C.N.,Weihe, C.D. (deposition date: 2018-08-08, release date: 2018-08-22, Last modification date: 2024-01-17)

Primary citation

Lo Leggio, L.,Weihe, C.D.,Poulsen, J.N.,Sweeney, M.,Rasmussen, F.,Lin, J.,De Maria, L.,Wogulis, M.
Structure of a lytic polysaccharide monooxygenase from Aspergillus fumigatus and an engineered thermostable variant.
Carbohydr. Res., 469:55-59, 2018

PubMed Abstract: Lytic polysaccharide monooxygenases (LPMOs) are industrial enzymes which are gaining use in second generation bioethanol production from lignocellulose by acting in synergy with glycoside hydrolases. Here we present the X-ray crystal structure of an AA9 fungal LPMO from Aspergillus fumigatus and a variant which has been shown to have better performance at elevated temperatures. Based on the structures, thermal denaturation data and theoretical calculations, we provide a suggestion for the structural basis of the improved stability.

PubMed: 30296642
DOI: 10.1016/j.carres.2018.08.009

Experimental method

X-RAY DIFFRACTION (1.37 Å)