6H79

SSX structure of Lysozyme in flow - metal-kapton microfluidic device

Summary for 6H79

• Entry DOI: 10.2210/pdb6h79/pdb
• Descriptor: Lysozyme C, CHLORIDE ION, ACETATE ION, ... (6 entities in total)
• Functional Keywords: hydrolase
• Biological source: Gallus gallus (Chicken)
• Total number of polymer chains: 1
• Total formula weight: 14510.71

Authors

Monteiro, D.C.F.,Trebbin, M. (deposition date: 2018-07-30, release date: 2019-03-20, Last modification date: 2024-10-16)

Primary citation

Monteiro, D.C.F.,Vakili, M.,Harich, J.,Sztucki, M.,Meier, S.M.,Horrell, S.,Josts, I.,Trebbin, M.
A microfluidic flow-focusing device for low sample consumption serial synchrotron crystallography experiments in liquid flow.
J.Synchrotron Radiat., 26:406-412, 2019

PubMed Abstract: Serial synchrotron crystallography allows low X-ray dose, room-temperature crystal structures of proteins to be determined from a population of microcrystals. Protein production and crystallization is a non-trivial procedure and it is essential to have X-ray-compatible sample environments that keep sample consumption low and the crystals in their native environment. This article presents a fast and optimized manufacturing route to metal-polyimide microfluidic flow-focusing devices which allow for the collection of X-ray diffraction data in flow. The flow-focusing conditions allow for sample consumption to be significantly decreased, while also opening up the possibility of more complex experiments such as rapid mixing for time-resolved serial crystallography. This high-repetition-rate experiment allows for full datasets to be obtained quickly (∼1 h) from crystal slurries in liquid flow. The X-ray compatible microfluidic chips are easily manufacturable, reliable and durable and require sample-flow rates on the order of only 30 µl h.

PubMed: 30855249
DOI: 10.1107/S1600577519000304

Experimental method

X-RAY DIFFRACTION (2.1 Å)