6H3B
Lysozyme: Machining protein microcrystals for structure determination by electron diffraction
Summary for 6H3B
| Entry DOI | 10.2210/pdb6h3b/pdb |
| Descriptor | Lysozyme C, CHLORIDE ION (3 entities in total) |
| Functional Keywords | lysozyme, hydrolase |
| Biological source | Gallus gallus (Chicken) |
| Total number of polymer chains | 1 |
| Total formula weight | 14366.61 |
| Authors | Duyvesteyn, H.M.E.,Ginn, H.M.,Stuart, D.I. (deposition date: 2018-07-18, release date: 2018-09-12, Last modification date: 2024-11-13) |
| Primary citation | Duyvesteyn, H.M.E.,Kotecha, A.,Ginn, H.M.,Hecksel, C.W.,Beale, E.V.,de Haas, F.,Evans, G.,Zhang, P.,Chiu, W.,Stuart, D.I. Machining protein microcrystals for structure determination by electron diffraction. Proc. Natl. Acad. Sci. U.S.A., 115:9569-9573, 2018 Cited by PubMed Abstract: We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffraction and micron scale of synchrotron microfocus beamlines. The demonstration that atomic information can be retained suggests that milling could provide such detail on sections cut from vitrified cells. PubMed: 30171169DOI: 10.1073/pnas.1809978115 PDB entries with the same primary citation |
| Experimental method | ELECTRON CRYSTALLOGRAPHY (1.9 Å) |
Structure validation
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