6GZN

Adenine-driven structural switch from two- to three-quartet DNA G-quadruplex

Summary for 6GZN

• Entry DOI: 10.2210/pdb6gzn/pdb
• NMR Information: BMRB: 34297
• Descriptor: DNA (5'-D(*GP*GP*GP*TP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*AP*GP*GP*G)-3') (1 entity in total)
• Functional Keywords: g-quadruplex, basket-type topology, rankl gene, osteoporosis, dna
• Biological source: Homo sapiens
• Total number of polymer chains: 1
• Total formula weight: 6410.13

Authors

Lenarcic Zivkovic, M.,Rozman, J.,Plavec, J. (deposition date: 2018-07-04, release date: 2018-09-26, Last modification date: 2024-05-15)

Primary citation

Lenarcic Zivkovic, M.,Rozman, J.,Plavec, J.
Adenine-Driven Structural Switch from a Two- to Three-Quartet DNA G-Quadruplex.
Angew. Chem. Int. Ed. Engl., 57:15395-15399, 2018

PubMed Abstract: A G-rich sequence found in the regulatory region of the RANKL gene, which is associated with homeostasis of bone metabolism, folds into a two-quartet basket-type G-quadruplex stabilized by A⋅G⋅A and G⋅G⋅G base-triads. Perusal of local structural features together with G/A-to-T modifications uncovered the critical role of A5 for the formation of a distinct antiparallel two-quartet topology and not the three-quartet topology that would be expected based on the sequence with four GGG-tracts alone. The structural changes induced by the A5-to-T5 modification include a switch in orientation and relative positions of G-strands that together with anti to syn reorientation of G12 provide insights into the complexity of the interactions that influence the folding of G-rich DNA. Understanding the impact of loop residues on the stability and formation of G-quadruplexes advances our knowledge and ability to predict structures adopted by G-rich sequences, which are involved in regulatory mechanisms in the cell, and may also facilitate drug design.

PubMed: 30222243
DOI: 10.1002/anie.201809328

Experimental method

SOLUTION NMR