6GZ4

tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-2 (TI-POST-2)

This is a large structure.

Summary for 6GZ4

Related6GZ3 6GZ5
EMDB information0100 0098 0099
Descriptorribosomal protein uL2, ribosomal protein uS2, ribosomal protein uL3, ... (89 entities in total)
Functional Keywordstranslocation, mammalian, 80s, eef2, ef-g, gmppnp, dde, diphthamide, ribosome
Biological sourceOryctolagus cuniculus
Total number of polymer chains86
Total molecular weight3325454.94
Authors
Flis, J.,Holm, M.,Rundlet, E.J.,Loerke, J.,Hilal, T.,Dabrowski, M.,Buerger, J.,Mielke, T.,Blanchard, S.C.,Spahn, C.M.T.,Budkevich, T.V. (deposition date: 2018-07-03, release date: 2018-12-05, Last modification date: 2019-02-06)
Primary citation
Flis, J.,Holm, M.,Rundlet, E.J.,Loerke, J.,Hilal, T.,Dabrowski, M.,Burger, J.,Mielke, T.,Blanchard, S.C.,Spahn, C.M.T.,Budkevich, T.V.
tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis.
Cell Rep, 25:2676-2688.e7, 2018
PubMed: 30517857 (PDB entries with the same primary citation)
DOI: 10.1016/j.celrep.2018.11.040
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone6 0.2% 0.9% 0.52MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 6gz4
no rotation
Molmil generated image of 6gz4
rotated about x axis by 90°
Molmil generated image of 6gz4
rotated about y axis by 90°
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