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- EMDB-0100: tRNA translocation by the eukaryotic 80S ribosome and the impact ... -

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Basic information

Entry
Database: EMDB / ID: EMD-0100
TitletRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-3 (TI-POST-3)
Map data
Sample
  • Complex: translocation intermediate TI-POST-3
    • Complex: translocation intermediate TI-POST-3
      • RNA: x 7 types
      • Protein or peptide: x 78 types
    • Complex: translocation intermediate TI-POST-3
      • Protein or peptide: x 1 types
    • Complex: translocation intermediate TI-POST-3
  • Ligand: x 3 types
Biological speciesOryctolagus cuniculus (rabbit) / Enterobacteria phage SP6 (virus) / Saccharomyces cerevisiae (brewer's yeast) / rabbit (rabbit) / baker's yeast (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsFlis J / Holm M / Rundlet EJ / Loerke J / Hilal T / Dabrowski M / Buerger J / Mielke T / Blanchard SC / Spahn CMT / Budkevich TV
Funding support Germany, United States, 3 items
OrganizationGrant numberCountry
German Research FoundationDFG SFB 740 Germany
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM079238 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)T32 GM115327-Tan United States
CitationJournal: Cell Rep / Year: 2018
Title: tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis.
Authors: Julia Flis / Mikael Holm / Emily J Rundlet / Justus Loerke / Tarek Hilal / Marylena Dabrowski / Jörg Bürger / Thorsten Mielke / Scott C Blanchard / Christian M T Spahn / Tatyana V Budkevich /
Abstract: Translocation moves the tRNA⋅mRNA module directionally through the ribosome during the elongation phase of protein synthesis. Although translocation is known to entail large conformational changes ...Translocation moves the tRNA⋅mRNA module directionally through the ribosome during the elongation phase of protein synthesis. Although translocation is known to entail large conformational changes within both the ribosome and tRNA substrates, the orchestrated events that ensure the speed and fidelity of this critical aspect of the protein synthesis mechanism have not been fully elucidated. Here, we present three high-resolution structures of intermediates of translocation on the mammalian ribosome where, in contrast to bacteria, ribosomal complexes containing the translocase eEF2 and the complete tRNA⋅mRNA module are trapped by the non-hydrolyzable GTP analog GMPPNP. Consistent with the observed structures, single-molecule imaging revealed that GTP hydrolysis principally facilitates rate-limiting, final steps of translocation, which are required for factor dissociation and which are differentially regulated in bacterial and mammalian systems by the rates of deacyl-tRNA dissociation from the E site.
History
DepositionJul 3, 2018-
Header (metadata) releaseOct 24, 2018-
Map releaseDec 5, 2018-
UpdateMar 30, 2022-
Current statusMar 30, 2022Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 2.5
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 2.5
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-6gz5
  • Surface level: 2.5
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_0100.map.gz / Format: CCP4 / Size: 253.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.975 Å
Density
Contour LevelBy AUTHOR: 2.5 / Movie #1: 2.5
Minimum - Maximum-4.1486583 - 13.064281
Average (Standard dev.)0.34266785 (±0.69080204)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions405405405
Spacing405405405
CellA=B=C: 394.875 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.9750.9750.975
M x/y/z405405405
origin x/y/z0.0000.0000.000
length x/y/z394.875394.875394.875
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ450450450
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS405405405
D min/max/mean-4.14913.0640.343

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Supplemental data

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Sample components

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Entire : translocation intermediate TI-POST-3

EntireName: translocation intermediate TI-POST-3
Components
  • Complex: translocation intermediate TI-POST-3
    • Complex: translocation intermediate TI-POST-3
      • RNA: 28S ribosomal RNA
      • RNA: P/P-site-tRNA
      • RNA: mRNAMessenger RNA
      • RNA: E/E-site-tRNA
      • RNA: 18S ribosomal RNA
      • Protein or peptide: ribosomal protein uS3
      • Protein or peptide: ribosomal protein uS7
      • Protein or peptide: ribosomal protein eS10
      • Protein or peptide: ribosomal protein eS12
      • Protein or peptide: ribosomal protein uS19
      • Protein or peptide: ribosomal protein uS9
      • Protein or peptide: ribosomal protein eS17
      • Protein or peptide: ribosomal protein uS13
      • Protein or peptide: ribosomal protein eS19
      • Protein or peptide: ribosomal protein uS10
      • Protein or peptide: ribosomal protein eS25
      • Protein or peptide: ribosomal protein eS28
      • Protein or peptide: ribosomal protein eS31
      • Protein or peptide: ribosomal protein RACK 1Ribosome
      • Protein or peptide: ribosomal protein uS2
      • Protein or peptide: ribosomal protein eS1
      • Protein or peptide: ribosomal protein uS5
      • Protein or peptide: ribosomal protein eS4
      • Protein or peptide: ribosomal protein eS6
      • Protein or peptide: ribosomal protein eS7
      • Protein or peptide: ribosomal protein eS8
      • Protein or peptide: ribosomal protein uS4
      • Protein or peptide: ribosomal protein uS17
      • Protein or peptide: ribosomal protein uS15
      • Protein or peptide: ribosomal protein uS11
      • Protein or peptide: ribosomal protein eS21
      • Protein or peptide: ribosomal protein uS8
      • Protein or peptide: ribosomal protein uS12
      • Protein or peptide: ribosomal protein eS24
      • Protein or peptide: ribosomal protein eS26
      • Protein or peptide: ribosomal protein eS27
      • Protein or peptide: ribosomal protein eS30
      • RNA: 5.8S ribosomal RNA
      • RNA: 5S ribosomal RNA
      • Protein or peptide: ribosomal protein uL2
      • Protein or peptide: ribosomal protein uL3
      • Protein or peptide: ribosomal protein uL4
      • Protein or peptide: ribosomal protein uL18
      • Protein or peptide: ribosomal protein eL6
      • Protein or peptide: ribosomal protein uL30
      • Protein or peptide: ribosomal protein eL8
      • Protein or peptide: ribosomal protein uL6
      • Protein or peptide: ribosomal protein uL16
      • Protein or peptide: ribosomal protein uL5
      • Protein or peptide: ribosomal protein eL13
      • Protein or peptide: ribosomal protein eL14
      • Protein or peptide: ribosomal protein eL15
      • Protein or peptide: ribosomal protein uL13
      • Protein or peptide: ribosomal protein uL22
      • Protein or peptide: ribosomal protein eL18
      • Protein or peptide: ribosomal protein eL19
      • Protein or peptide: ribosomal protein eL20
      • Protein or peptide: ribosomal protein eL21
      • Protein or peptide: ribosomal protein eL22
      • Protein or peptide: ribosomal protein uL14
      • Protein or peptide: ribosomal protein eL24
      • Protein or peptide: ribosomal protein uL23
      • Protein or peptide: ribosomal protein uL24
      • Protein or peptide: ribosomal protein eL27
      • Protein or peptide: ribosomal protein uL15
      • Protein or peptide: ribosomal protein eL29
      • Protein or peptide: ribosomal protein eL30
      • Protein or peptide: ribosomal protein eL31
      • Protein or peptide: ribosomal protein eL32
      • Protein or peptide: ribosomal protein eL33
      • Protein or peptide: ribosomal protein eL34
      • Protein or peptide: ribosomal protein uL29
      • Protein or peptide: ribosomal protein eL36
      • Protein or peptide: ribosomal protein eL37
      • Protein or peptide: ribosomal protein eL38
      • Protein or peptide: ribosomal protein eL39
      • Protein or peptide: ribosomal protein eL40
      • Protein or peptide: ribosomal protein eL41
      • Protein or peptide: ribosomal protein eL42
      • Protein or peptide: ribosomal protein eL43
      • Protein or peptide: ribosomal protein eL28
      • Protein or peptide: ribosomal protein uL1
      • Protein or peptide: ribosomal protein uL11
      • Protein or peptide: ribosomal protein uL10
      • Protein or peptide: eukaryotic elongation factor 2 (eEF2)
    • Complex: translocation intermediate TI-POST-3
      • Protein or peptide: ribosomal protein uS14
    • Complex: translocation intermediate TI-POST-3
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

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Supramolecule #1: translocation intermediate TI-POST-3

SupramoleculeName: translocation intermediate TI-POST-3 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#86
Molecular weightTheoretical: 4.2 MDa

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Supramolecule #2: translocation intermediate TI-POST-3

SupramoleculeName: translocation intermediate TI-POST-3 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#17, #19-#86
Source (natural)Organism: Oryctolagus cuniculus (rabbit)

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Supramolecule #3: translocation intermediate TI-POST-3

SupramoleculeName: translocation intermediate TI-POST-3 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #3, #18
Source (natural)Organism: Enterobacteria phage SP6 (virus)
Recombinant expressionOrganism: Enterobacteria phage SP6 (virus)

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Supramolecule #4: translocation intermediate TI-POST-3

SupramoleculeName: translocation intermediate TI-POST-3 / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #4
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Macromolecule #1: 28S ribosomal RNA

MacromoleculeName: 28S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 1.170164875 MDa
SequenceString: CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGGGCG CGGGACAUGU GGCGUACGGA A GACCCGCU ...String:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGGGCG CGGGACAUGU GGCGUACGGA A GACCCGCU CCCCGGCGCC GCUCGUGGGG GGCCCAAGUC CUUCUGAUCG AGGCCCAGCC CGUGGACGGU GUGAGGCCGG UA GCGGCCC CCGGCGCGCG CCCGGGUCUU CCCGGAGUCG GGUUGCUUGG GAAUGCAGCC CAAAGCGGGU GGUAAACUCC AUC UAAGGC UAAAUACCGG CACGAGACCG AUAGUCAACA AGUACCGUAA GGGAAAGUUG AAAAGAACUU UGAAGAGAGA GUUC AAGAG GGCGUGAAAC CGUUAAGAGG UAAACGGGUG GGGUCCGCGC AGUCCGCCCG GAGGAUUCAA CCCGGCGGCG GGUCC GGCC GUGUCGGCGG CCCGGCGGAU CUUUCCCGCC CCCGGGGUCG GCGGGGGACC GUCCCCCGGA CCGGCGACCG GCCGCC GCC GGGCGCAUUU CCAGGCGGUG CGCCGCGACC GGCUCCGGGA CGGCUGGGAA GGCCCGGCGG GGAAGGUGGC UCGGGGC CC CCGAGUGUUA CAGCCCCCCC GGCAGCAGCA CUCGCCGAAU CCCGGGGCCG AGGGAGCGAG ACCCGUCGCC GCGCUCUC C CCCCUGGGGG GGCCGGGCCA CCCCUCCCAC GGCGCGACCG CUCUCCCAGG GGGCGGGGCG GACUGUCCCC AGUGCGCCC CGGGCGGGUC GCGCCGUCGG GCCCGGGGGG CCACGCGCGC GUCCCGGGAC GGCGGAGCGA GCGCACGGGG UCGGCGGCGA CGUCGGCUA CCCACCCGAC CCGUCUUGAA ACACGGACCA AGGAGUCUAA CACGUGCGCG AGUCGGGGGC UCGCACGAAA G CCGCCGUG GCGCAAUGAA GGUGAAGGCC GGCGCGCUCG CCGGCCGAGG UGGGAUCCCG AGGCCUCUCC AGUCCGCCGA GG GGCACCA CCGGCCCGUC UCGCCCGCCG CGCCGGGGAG GUGGAGCACG AGCGCACGUG UUAGGACCCG AAAGAUGGUG AAC UAUGCC UGGGCAGGGC GAAGCCAGAG GAAACUCUGG UGGAGGUCCG UAGCGGUCCU GACGUGCAAA UCGGUCGUCC GACC UGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGUU CCCUCCGAAG UUUCCCUCAG GAUAGCUGGC GCUCU CGCC CACGCAGUUU UAUCCGGUAA AGCGAAUGAU UAGAGGUCUU GGGGCCGAAA CGAUCUCAAC CUAUUCUCAA ACUUUA AAU GGGUAAGAAG CCCGGCUCGC UGGCGUGGAG CCGGGGUGGA AUGCGAGUGC CUAGUGGGCC ACUUUUGGUA AGCAGAA CU GGCGCUGCGG GAUGAACCGA ACGCCGGGUU AAGGCGCCCG AUGCCGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUU G AUAUAGACAG CAGGACGGUG GCCAUGGAAG UCGGAAUCCG CUAAGGAGUG UGUAACAACU CACCUGCCGA AUCAACUAG CCCUGAAAAU GGAUGGCGCU GGAGCGUCGG GCCCAUACCC GGCCGUCGCC GGCCUACGCC GCGACGAGUA GGAGGGCCGC UGCGGUGAG CCUUGAAGCC UAGGGCGCGG GCCCGGGUGG AGCCGCCGCA GGUGCAGAUC UUGGUGGUAG UAGCAAAUAU U CAAACGAG AACUUUGAAG GCCGAAGUGG AGAAGGGUUC CAUGUGAACA GCAGUUGAAC AUGGGUCAGU CGGUCCUGAG AG AUGGGCG AGCGCCGUUC CGAAGGGACG GGCGAUGGCC UCCGUUGCCC UCGGCCGAUC GAAAGGGAGU CGGGUUCAGA UCC CCGAAU CCGGAGUGGC GGAGAUGGGC GCCGCGAGGC GUCCAGUGCG GUAACGCGAC CGAUCCCGGA GAAGCCGGCG GGAG CCCCG GGGAGAGUUC UCUUUUCUUU GUGAAGGGCA GGGCGCCCUG GAAUGGGUUC GCCCCGAGAG AGGGGCCCGU GCCUU GGAA AGCGUCGCGG UUCCGGCGGC GUCCGGUGAG CUCUCGCUGG CCCUUGAAAA UCCGGGGGAG AGGGUGUAAA UCUCGC GCC GGGCCGUACC CAUAUCCGCA GCAGGUCUCC AAGGUGAACA GCCUCUGGCA UGUUGGAACA AUGUAGGUAA GGGAAGU CG GCAAGCCGGA UCCGUAACUU CGGGAUAAGG AUUGGCUCUA AGGGCUGGCA GCCGACUUAG AACUGGUGCG GACCAGGG G AAUCCGACUG UUUAAUUAAA ACAAAGCAUC GCGAAGGCCC GCGGCGGGUG UUGACGCGAU GUGAUUUCUG CCCAGUGCU CUGAAUGUCA AAGUGAAGAA AUUCAAUGAA GCGCGGGUAA ACGGCGGGAG UAACUAUGAC UCUCUUAAGG UAGCCAAAUG CCUCGUCAU CUAAUUAGUG ACGCGCAUGA AUGGAUGAAC GAGAUUCCCA CUGUCCCUAC CUACUAUCCA GCGAAACCAC A GCCAAGGG AACGGGCUUG GCGGAAUCAG CGGGGAAAGA AGACCCUGUU GAGCUUGACU CUAGUCUGGC ACGGUGAAGA GA CAUGAGA GGUGUAGAAU AAGUGGGAGG CCCGGGCCGC CGGUGAAAUA CCACUACUCU GAUCGUUUUU UCACUGACCC GGU GAGGCG GGGGGGCGAG CCCGAGGGGC UCUCGCUUCU GGCGCCAAGC GCCCGCCCGG CCGGGCGCGA CCCGCUCCGG GGAC AGUGC CAGGUGGGGA GUUUGACUGG GGCGGUACAC CUGUCAAACG GUAACGCAGG UGUCCUAAGG CGAGCUCAGG GAGGA CAGA AACCUCCCGU GGAGCAGAAG GGCAAAAGCU CGCUUGAUCU UGAUUUUCAG UACGAAUACA GACCGUGAAA GCGGGG CCU CACGAUCCUU CUGACCUUUU GGGUUUUAAG CAGGAGGUGU CAGAAAAGUU ACCACAGGGA UAACUGGCUU GUGGCGG CC AAGCGUUCAU AGCGACGUCG CUUUUUGAUC CUUCGAUGUC GGCUCUUCCU AUCAUUGUGA AGCAGAAUUC GCCAAGCG U UGGAUUGUUC ACCCACUAAU AGGGAACGUG AGCUGGGUUU AGACCGUCGU GAGACAGGUU AGUUUUACCC UACUGAUGA UGUGUUGUUG CCAUGGUAAU CCUGCUCAGU ACGAGAGGAA CCGCAGGUUC AGACAUUUGG UGUAUGUGCU UGGCUGAGGA GCCAAUGGG GCGAAGCUAC CAUCUGUGGG AUUAUGACUG AACGCCUCUA AGUCAGAAUC CCGCCCAGGC GGAACGAUAC G GCAGCGCC GCGGAGCCUC GGUUGGCCUC GGAUAGCCGG UCCGGGGUCC GGUGCGGAGU GCCCUUCGUC CUGGGAAACG GG GCGCGGC CGGAAAGGCG GCCGCCCCCU CGCCCGUCAC GCACCGCACG UUCGUGGGGA ACCUGGCGCU AAACCAUUCG UAG ACGACC UGCUUCUGGG UCGGGGUUUC GUACGUAGCA GAGCAGCUCC CUCGCUGCGA UCUAUUGAAA GUCAGCCCUC GACA CAAGG GUUUGUC

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Macromolecule #2: P/P-site-tRNA

MacromoleculeName: P/P-site-tRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.437535 KDa
SequenceString:
GUUUCCGUAG UGUAGUGGUU AUCACGUUCG CCUAACACGC GAAAGGUCCC CGGUUCGAAA CCGGGCGGAA ACACCA

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Macromolecule #3: mRNA

MacromoleculeName: mRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Enterobacteria phage SP6 (virus)
Molecular weightTheoretical: 3.492122 KDa
SequenceString:
UUCGUUAAAG A

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Macromolecule #4: E/E-site-tRNA

MacromoleculeName: E/E-site-tRNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 24.533586 KDa
SequenceString:
GCGGACUUAG CUCAGUUGGG AGAGCGCCAG ACUGAAGAUC UGGAGGUCCU GUGUUCGAUC CACAGAGUUC GCACCA

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Macromolecule #5: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 551.108188 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGACU UGGAUAACUG UGGUAAUUCU AGAGCUAAUA C AUGCCGAC ...String:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGACU UGGAUAACUG UGGUAAUUCU AGAGCUAAUA C AUGCCGAC GGGCGCUGAC CCCCUUCGCG GGGGGGAUGC GUGCAUUUAU CAGAUCAAAA CCAACCCGGG CUUUGGUGAC UC UAGAUAA CCUCGGGCCG AUCGCACGCC CCCCGUGGCG GCGACGACCC AUUCGAACGU CUGCCCUAUC AACUUUCGAU GGU AGUCGC CGUGCCUACC AUGGUGACCA CGGGUGACGG GGAAUCAGGG UUCGAUUCCG GAGAGGGAGC CUGAGAAACG GCUA CCACA UCCAAGGAAG GCAGCAGGCG CGCAAAUUAC CCACUCCCGA CCCGGGGAGG UAGUGACGAA AAAUAACAAU ACAGG ACUC UUUCGAGGCC CUGUAAUUGG AAUGAGUCCA CUUUAAAUCC UUUAACGAGG AUCCAUUGGA GGGCAAGUCU GGUGCC AGC AGCCGCGGUA AUUCCAGCUC CAAUAGCGUA UAUUAAAGUU GCUGCAGUUA AAAAGCUCGU AGUUGGAUCU UGGGAGC GG CCCCUUGCCU CUCGCCCGAA GCGUUUACUU UGAAAAAAUU AGAGUGUUCA AAGCAGGCCC GAGCCGCCUG GAUACCGC A GCUAGGAAUA AUGGAAUAGG ACCGCGGUUC UAUUUUGUUG GUUUUCGGAA CUGAGGCCAU GAUUAAGAGG GACGGCCGG GGGCAUUCGU AUUGCGCCGC UAGAGGUGAA AUUCUUGGAC CGGCGCAAGA CGGACCAGAG CGAAAGCAUU UGCCAAGAAU GUUUUCAUU AAUCAAGAAC GAAAGUCGGA GGUUCGAAGA CGAUCAGAUA CCGUCGUAGU UCCGACCAUA AACGAUGCCG A CCGGCGAU GCGGCGGCGU UAUUCCCAUG ACCCGCCGGG CAGCUUCCGG GAAACCAAAG UCUUUGGGUU CCGGGGGGAG UA UGGUUGC AAAGCUGAAA CUUAAAGGAA UUGACGGAAG GGCACCACCA GGAGUGGAGC CUGCGGCUUA AUUUGACUCA ACA CGGGAA ACCUCACCCG GCCCGGACAC GGACAGGAUU GACAGAUUGA UAGCUCUUUC UCGAUUCCGU GGGUGGUGGU GCAU GGCCG UUCUUAGUUG GUGGAGCGAU UUGUCUGGUU AAUUCCGAUA ACGAACGAGA CUCUGGCAUG CUAACUAGUU ACGCG ACCC CCGAGCGGUA ACUUCUUAGA GGGACAAGUG GCGUUCAGCC ACCCGAGAUU GAGCAAUAAC AGGUCUGUGA UGCCCU UAG AUGUCCGGGG CUGCACGCGC GCUACACUGA CUGGCUCAGC GUGUGCCUAC CCUACGCCGG CAGGCGCGGG UAACCCG UU GAACCCCAUU CGUGAUGGGG AUCGGGGAUU GCAAUUAUUC CCCAUGAACG AGGAAUUCCC AGUAAGUGCG GGUCAUAA G CUUGCGUUGA UUAAGUCCCU GCCCUUUGUA CACACCGCCC GUCGCUACUA CCGAUUGGAU GGUUUAGUGA GGCCCUCGG AUCGGCCCCG CCGUGGCGGA GCGCUGAGAA GACGGUCGAA CUUGACUAUC UAGAGGAAGU AAAAGUCGUA ACAAGGUUUC CGUAGGUGA ACCUGCGGAA GGAUCAUUA

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Macromolecule #39: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 39 / Number of copies: 1
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 50.449812 KDa
SequenceString:
CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCUU

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Macromolecule #40: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 40 / Number of copies: 1
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 38.38575 KDa
SequenceString:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU

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Macromolecule #6: ribosomal protein uS3

MacromoleculeName: ribosomal protein uS3 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.35759 KDa
SequenceString: AVQISKKRKF VADGIFKAEL NEFLTRELAE DGYSGVEVRV TPTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEK VATRGLCAIA QAESLRYKLL GGLAVRRACY GVLRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS G DPVNYYVD ...String:
AVQISKKRKF VADGIFKAEL NEFLTRELAE DGYSGVEVRV TPTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEK VATRGLCAIA QAESLRYKLL GGLAVRRACY GVLRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS G DPVNYYVD TAVRHVLLRQ GVLGIKVKIM LPWDPTGKIG PKKPLPDHVS IVEPKDEILP TT

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Macromolecule #7: ribosomal protein uS7

MacromoleculeName: ribosomal protein uS7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 21.424836 KDa
SequenceString:
PDIKLFGKWS TDDVQINDIS LQDYIAVKEK YAKYLPHSAG RYAAKRFRKA QCPIVERLTN SMMMHGRNNG KKLMTVRIVK HAFEIIHLL TGENPLQVLV NAIINSGPRE DSTRIGRAGT VRRQAVDVSP LRRVNQAIWL LCTGAREAAF RNIKTIAECL A DELINAAK GSSNSYAIKK KDELERVAKS NR

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Macromolecule #8: ribosomal protein eS10

MacromoleculeName: ribosomal protein eS10 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 11.773953 KDa
SequenceString:
MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSR

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Macromolecule #9: ribosomal protein eS12

MacromoleculeName: ribosomal protein eS12 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 13.393658 KDa
SequenceString:
VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWVG LCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK C

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Macromolecule #10: ribosomal protein uS19

MacromoleculeName: ribosomal protein uS19 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 14.273879 KDa
SequenceString:
FRKFTYRGVD LDQLLDMSYE QLMQLYSARQ RRRLNRGLRR KQHSLLKRLR KAKKEAPPME KPEVVKTHLR DMIILPEMVG SMVGVYNGK TFNQVEIKPE MIGHYLGEFS ITYKPVKHGR P

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Macromolecule #11: ribosomal protein uS9

MacromoleculeName: ribosomal protein uS9 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 15.76548 KDa
SequenceString:
QSVQVFGRKK TATAVAHCKR GNGLIKVNGR PLEMIEPRTL QYKLLEPVLL LGKERFAGVD IRVRVKGGGH VAQIYAIRQS ISKALVAYY QKYVDEASKK EIKDILIQYD RTLLVADPRR CESKKFGGPG ARARYQKSYR

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Macromolecule #12: ribosomal protein eS17

MacromoleculeName: ribosomal protein eS17 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 14.447793 KDa
SequenceString:
GRVRTKTVKK AARVIIEKYY TRLGNDFHTN KRVCEEIAII PSKKLRNKIA GYVTHLMKRI QRGPVRGISI KLQEEERERR DNYVPEVSA LDQEIIEVDP DTKEMLKLLD FGSLSNLQVT QPTVGM

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Macromolecule #13: ribosomal protein uS13

MacromoleculeName: ribosomal protein uS13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 16.383064 KDa
SequenceString:
VIPEKFQHIL RVLNTNIDGR RKIAFAITAI KGVGRRYAHV VLRKADIDLT KRAGELTEDE VERVITIMQN PRQYKIPDWF LNRQKDVKD GKYSQVLANG LDNKLREDLE RLKKIRAHRG LRHFWGLRVR GQHTKTTGRR

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Macromolecule #14: ribosomal protein eS19

MacromoleculeName: ribosomal protein eS19 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 15.822219 KDa
SequenceString:
PGVTVKDVNQ QEFVRALAAF LKKSGKLKVP EWVDTVKLAK HKELAPYDEN WFYTRAASTA RHLYLRGGAG VGSMTKIYGG RQRNGVMPS HFSRGSKSVA RRVLQALEGL KMVEKDQDGG RKLTPQGQRD LDRIAGQVAA ANKK

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Macromolecule #15: ribosomal protein uS10

MacromoleculeName: ribosomal protein uS10 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 11.029072 KDa
SequenceString:
IRITLTSRNV KSLEKVCADL IRGAKEKNLK VKGPVRMPTK TLRITTRKTP CGEGSKTWDR FQMRIHKRLI DLHSPSEIVK QITSISIEP GVEVEVTI

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Macromolecule #16: ribosomal protein eS25

MacromoleculeName: ribosomal protein eS25 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 9.801748 KDa
SequenceString:
GKAKKKKWSK GKVRDKLNNL VLFDKATYDK LCKEVPNYKL ITPAVVSERL KIRGSLARAA LQELLSKGLI KLVSKHRAQV IYTRNT

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Macromolecule #17: ribosomal protein eS28

MacromoleculeName: ribosomal protein eS28 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 7.007069 KDa
SequenceString:
QPIKLARVTK VLGRTGSQGQ CTQVRVEFMD DTSRSIIRNV KGPVREGDVL TLLESEREAR RL

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Macromolecule #18: ribosomal protein uS14

MacromoleculeName: ribosomal protein uS14 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterobacteria phage SP6 (virus)
Molecular weightTheoretical: 6.12121 KDa
Recombinant expressionOrganism: Enterobacteria phage SP6 (virus)
SequenceString:
QLYWSHPRKF GQGSRSCRVC SNRHGLIRKY GLNMCRQCFR QYAKDIGFIK L

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Macromolecule #19: ribosomal protein eS31

MacromoleculeName: ribosomal protein eS31 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 8.645277 KDa
SequenceString:
KRKKKSYTTP KKNKHKRKKV KLAVLKYYKV DENGKISRLR RECPSDECGA GVFMASHFDR HYCGKCCLTY CFN

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Macromolecule #20: ribosomal protein RACK 1

MacromoleculeName: ribosomal protein RACK 1 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 34.726164 KDa
SequenceString: TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG ...String:
TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG WDKLVKVWNL ANCKLKTNHI GHTGYLNTVT VSPDGSLCAS GGKDGQAMLW DLNEGKHLYT LDGGDIINAL CF SPNRYWL CAATGPSIKI WDLEGKIIVD ELKQEVISTS SKAEPPQCTS LAWSADGQTL FAGYTDNLVR VWQVTIG

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Macromolecule #21: ribosomal protein uS2

MacromoleculeName: ribosomal protein uS2 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.24574 KDa
SequenceString: LDVLQMKEED VLKFLAAGTH LGGTNLDFQM EQYIYKRKSD GIYIINLKRT WEKLLLAARA IVAIENPADV SVISSRNTGQ RAVLKFAAA TGATPIAGRF TPGTFTNQIQ AAFREPRLLV VTDPRADHQP LTEASYVNLP TIALCNTDSP LRYVDIAIPC N NKGAHSVG ...String:
LDVLQMKEED VLKFLAAGTH LGGTNLDFQM EQYIYKRKSD GIYIINLKRT WEKLLLAARA IVAIENPADV SVISSRNTGQ RAVLKFAAA TGATPIAGRF TPGTFTNQIQ AAFREPRLLV VTDPRADHQP LTEASYVNLP TIALCNTDSP LRYVDIAIPC N NKGAHSVG LMWWMLAREV LRMRGTISRE HPWEVMPDLY FYRDPEEIEK EEQAAAE

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Macromolecule #22: ribosomal protein eS1

MacromoleculeName: ribosomal protein eS1 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.660012 KDa
SequenceString: VDPFSKKDWY DVKAPAMFNI RNIGKTLVTR TQGTKIASDG LKGRVFEVSL ADLQNDEVAF RKFKLITEDV QGKNCLTNFH GMDLTRDKM CSMVKKWQTM IEAHVDVKTT DGYLLRLFCV GFTKKRNNQI RKTSYAQHQQ VRQIRKKMME IMTREVQTND L KEVVNKLI ...String:
VDPFSKKDWY DVKAPAMFNI RNIGKTLVTR TQGTKIASDG LKGRVFEVSL ADLQNDEVAF RKFKLITEDV QGKNCLTNFH GMDLTRDKM CSMVKKWQTM IEAHVDVKTT DGYLLRLFCV GFTKKRNNQI RKTSYAQHQQ VRQIRKKMME IMTREVQTND L KEVVNKLI PDSIGKDIEK ACQSIYPLHD VFVRKVKMLK KPKFELGKLM ELHG

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Macromolecule #23: ribosomal protein uS5

MacromoleculeName: ribosomal protein uS5 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.574971 KDa
SequenceString: DKEWMPVTKL GRLVKDMKIK SLEEIYLFSL PIKESEIIDF FLGASLKDEV LKIMPVQKQT RAGQRTRFKA FVAIGDYNGH VGLGVKCSK EVATAIRGAI ILAKLSIVPV RRGYWGNKIG KPHTVPCKVT GRCGSVLVRL IPAPRGTGIV SAPVPKKLLM M AGIDDCYT ...String:
DKEWMPVTKL GRLVKDMKIK SLEEIYLFSL PIKESEIIDF FLGASLKDEV LKIMPVQKQT RAGQRTRFKA FVAIGDYNGH VGLGVKCSK EVATAIRGAI ILAKLSIVPV RRGYWGNKIG KPHTVPCKVT GRCGSVLVRL IPAPRGTGIV SAPVPKKLLM M AGIDDCYT SARGCTATLG NFAKATFDAI SKTYSYLTPD LWKETVFTKS PYQEFTDHLV KTHT

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Macromolecule #24: ribosomal protein eS4

MacromoleculeName: ribosomal protein eS4 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 28.893043 KDa
SequenceString: ARGPKKHLKR VAAPKHWMLD KLTGVFAPRP STGPHKLREC LPLIVFLRNR LKYALTGDEV KKICMQRFIK IDGKVRVDVT YPAGFMDVI SIEKTGEHFR LVYDTKGRFA VHRITVEEAK YKLCKVRKIT VGVKGIPHLV THDARTIRYP DPVIKVNDTV Q IDLGTGKI ...String:
ARGPKKHLKR VAAPKHWMLD KLTGVFAPRP STGPHKLREC LPLIVFLRNR LKYALTGDEV KKICMQRFIK IDGKVRVDVT YPAGFMDVI SIEKTGEHFR LVYDTKGRFA VHRITVEEAK YKLCKVRKIT VGVKGIPHLV THDARTIRYP DPVIKVNDTV Q IDLGTGKI INFIKFDTGN LCMVIGGANL GRVGVITNRE RHPGSFDVVH VKDANGNSFA TRLSNIFVIG NGNKPWISLP RG KGIRLTV AEERDKRLAT

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Macromolecule #25: ribosomal protein eS6

MacromoleculeName: ribosomal protein eS6 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 26.913867 KDa
SequenceString: MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK ...String:
MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRR

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Macromolecule #26: ribosomal protein eS7

MacromoleculeName: ribosomal protein eS7 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 20.990465 KDa
SequenceString:
NGEKPDEFES GISQALLELE MNSDLKAQLR ELNITAAKEI EVGGGRKAII IFVPVPQLKS FQKIQVRLVR ELEKKFSGKH VVFIAQRRI LPKPTRKSRT KNKQKRPRSR TLTAVHDAIL EDLVFPSEIV GKRIRVKLDG SRLIKVHLDK AQQNNVEHKV E TFSGVYKK LTGKDVNFEF PEFQL

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Macromolecule #27: ribosomal protein eS8

MacromoleculeName: ribosomal protein eS8 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.132191 KDa
SequenceString: GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVRVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG ...String:
GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVRVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG KLLACIASRP GQCGRADGYV LEGKELEFYL RKIKARKGK

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Macromolecule #28: ribosomal protein uS4

MacromoleculeName: ribosomal protein uS4 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 21.222221 KDa
SequenceString:
PVARSWVCRK TYVTPRRPFE KSRLDQELKL IGEYGLRNKR EVWRVKFTLA KIRKAARELL TLDEKDPRRL FEGNALLRRL VRIGVLDEG KMKLDYILGL KIEDFLERRL QTQVFKLGLA KSIHHARVLI RQRHIRVRKQ VVNIPSFIVR LDSQKHIDFS L RSPYGGGR PGRVKRKNAK K

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Macromolecule #29: ribosomal protein uS17

MacromoleculeName: ribosomal protein uS17 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 17.910176 KDa
SequenceString:
TERAYQKQPT IFQNKKRVLL GETGKEKLPR YYKNIGLGFK TPKEAIEGTY IDKKCPFTGN VSIRGRILSG VVTKMKMQRT IVIRRDYLH YIRKYNRFEK RHKNMSVHLS PCFRDVQIGD IVTVGECRPL SKTVRFNVLK VTKAAGTKKQ FQKF

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Macromolecule #30: ribosomal protein uS15

MacromoleculeName: ribosomal protein uS15 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 17.057113 KDa
SequenceString:
GRMHAPGKGL SQSALPYRRS VPTWLKLTSD DVKEQIYKLA KKGLTPSQIG VILRDSHGVA QVRFVTGNKI LRILKSKGLA PDLPEDLYH LIKKAVAVRK HLERNRKDKD AKFRLILIES RIHRLARYYK TKRVLPPNWK YESSTASALV

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Macromolecule #31: ribosomal protein uS11

MacromoleculeName: ribosomal protein uS11 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 14.544659 KDa
SequenceString:
SLGPQVAEGE NVFGVCHIFA SFNDTFVHVT DLSGKETICR VTGGMKVKAD RDESSPYAAM LAAQDVAQRC KELGITALHI KLRATGGNR TKTPGPGAQS ALRALARSGM KIGRIEDVTP IPSDSTRRKG GRRGRRL

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Macromolecule #32: ribosomal protein eS21

MacromoleculeName: ribosomal protein eS21 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 8.863111 KDa
SequenceString:
MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS K

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Macromolecule #33: ribosomal protein uS8

MacromoleculeName: ribosomal protein uS8 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 14.734357 KDa
SequenceString:
VRMNVLADAL KSINNAEKRG KRQVLIRPCS KVIVRFLTVM MKHGYIGEFE IIDDHRAGKI VVNLTGRLNK CGVISPRFDV QLKDLEKWQ NNLLPSRQFG FIVLTTSAGI MDHEEARRKH TGGKILGFFF

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Macromolecule #34: ribosomal protein uS12

MacromoleculeName: ribosomal protein uS12 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 15.626392 KDa
SequenceString:
GKCRGLRTAR KLRSHRRDQK WHDKQYKKAH LGTALKANPF GGASHAKGIV LEKVGVEAKQ PNSAIRKCVR VQLIKNGKKI TAFVPNDGC LNFIEENDEV LVAGFGRKGH AVGDIPGVRF KVVKVANVSL LALYKGKKER PR

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Macromolecule #35: ribosomal protein eS24

MacromoleculeName: ribosomal protein eS24 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 14.489176 KDa
SequenceString:
TVTIRTRKFM TNRLLQRKQM VIDVLHPGKA TVPKTEIREK LAKMYKTTPD VIFVFGFRTH FGGGKTTGFG MIYDSLDYAK KNEPKHRLA RHGLYEKKKT SRKQRKERKN RMKKVRGTAK ANVGAG

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Macromolecule #36: ribosomal protein eS26

MacromoleculeName: ribosomal protein eS26 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 11.087117 KDa
SequenceString:
TKKRRNNGRA KKGRGHVQPI RCTNCARCVP KDKAIKKFVI RNIVEAAAVR DISEASVFDA YVLPKLYVKL HYCVSCAIHS KVVRNRSRE ARKDRTPP

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Macromolecule #37: ribosomal protein eS27

MacromoleculeName: ribosomal protein eS27 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 9.00057 KDa
SequenceString:
AKDLLHPSPE EEKRKHKKKR LVQSPNSYFM DVKCPGCYKI TTVFSHAQTV VLCVGCSTVL CQPTGGKARL TEGCSFRRKQ

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Macromolecule #38: ribosomal protein eS30

MacromoleculeName: ribosomal protein eS30 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 6.239447 KDa
SequenceString:
HGSLARAGKV RGQTPKVAKQ EKKKKKTGRA KRRMQYNRRF VNVVPTFGKK KGPNA

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Macromolecule #41: ribosomal protein uL2

MacromoleculeName: ribosomal protein uL2 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 27.456158 KDa
SequenceString: GRVIRGQRKG AGSVFRAHVK HRKGAARLRA VDFAERHGYI KGIVKDIIHD PGRGAPLAKV VFRDPYRFKK RTELFIAAEG IHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS A NRAVVGVV ...String:
GRVIRGQRKG AGSVFRAHVK HRKGAARLRA VDFAERHGYI KGIVKDIIHD PGRGAPLAKV VFRDPYRFKK RTELFIAAEG IHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS A NRAVVGVV AGGGRIDKPI LKAGRAYHKY KAKRNCWPRV RGVAMNPVEH PFGGGNHQHI GKPSTIRRDA PAGRKVGLIA AR RTGRLRG TKTVQ

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Macromolecule #42: ribosomal protein uL3

MacromoleculeName: ribosomal protein uL3 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 45.222949 KDa
SequenceString: SHRKFSAPRH GSLGFLPRKR SSRHRGKVKS FPKDDPSKPV HLTAFLGYKA GMTHIVREVD RPGSKVNKKE VVEAVTIVET PPMVVVGIV GYVETPRGLR TFKTVFAEHI SDECKRRFYK NWHKSKKKAF TKYCKKWQDE DGKKQLEKDF SSMKKYCQVI R VIAHTQMR ...String:
SHRKFSAPRH GSLGFLPRKR SSRHRGKVKS FPKDDPSKPV HLTAFLGYKA GMTHIVREVD RPGSKVNKKE VVEAVTIVET PPMVVVGIV GYVETPRGLR TFKTVFAEHI SDECKRRFYK NWHKSKKKAF TKYCKKWQDE DGKKQLEKDF SSMKKYCQVI R VIAHTQMR LLPLRQKKAH LMEIQVNGGT VAEKLDWARE RLEQQVPVNQ VFGQDEMIDV IGVTKGKGYK GVTSRWHTKK LP RKTHRGL RKVACIGAWH PARVAFSVAR AGQKGYHHRT EINKKIYKIG QGYLIKDGKL IKNNASTDYD LSDKSINPLG GFV HYGEVT NDFVMLKGCV VGTKKRVLTL RKSLLVQTKR RALEKIDLKF IDTTSKFGHG RFQTMEEKKA FMGPLKKD

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Macromolecule #43: ribosomal protein uL4

MacromoleculeName: ribosomal protein uL4 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 41.171547 KDa
SequenceString: CARPLISVYS EKGESSGKNV TLPAVFKAPI RPDIVNFVHT NLRKNNRQPY AVSELAGHQT SAESWGTGRA VARIPRVRGG GTHRSGQGA FGNMCRGGRM FAPTKTWRRW HRRVNTTQKR YAICSALAAS ALPALVMSKG HRIEEVPELP LVVEDKVEGY K KTKEAVLL ...String:
CARPLISVYS EKGESSGKNV TLPAVFKAPI RPDIVNFVHT NLRKNNRQPY AVSELAGHQT SAESWGTGRA VARIPRVRGG GTHRSGQGA FGNMCRGGRM FAPTKTWRRW HRRVNTTQKR YAICSALAAS ALPALVMSKG HRIEEVPELP LVVEDKVEGY K KTKEAVLL LKKLKAWNDI KKVYASQRMR AGKGKMRNRR RIQRRGPCII YNEDNGIIKA FRNIPGITLL NVSKLNILKL AP GGHVGRF CIWTESAFRK LDELYGTWRK AASLKSNYNL PMHKMINTDL SRILKSPEIQ RALRAPRKKI HRRVLKKNPL KNL RIMLKL NPYAKTMRRN TILRQARNHK LRVDKAAAAA AALQAKS

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Macromolecule #44: ribosomal protein uL18

MacromoleculeName: ribosomal protein uL18 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 34.091371 KDa
SequenceString: VKVVKNKAYF KRYQVKFRRR REGKTDYYAR KRLVIQDKNK YNTPKYRMIV RVTNRDIICQ IAYARIEGDM IVCAAYAHEL PKYGVKVGL TNYAAAYCTG LLLARRLLNR FGMDKIYEGQ VEVTGDEYNV ESIDGQPGAF TCYLDAGLAR TTTGNKVFGA L KGAVDGGL ...String:
VKVVKNKAYF KRYQVKFRRR REGKTDYYAR KRLVIQDKNK YNTPKYRMIV RVTNRDIICQ IAYARIEGDM IVCAAYAHEL PKYGVKVGL TNYAAAYCTG LLLARRLLNR FGMDKIYEGQ VEVTGDEYNV ESIDGQPGAF TCYLDAGLAR TTTGNKVFGA L KGAVDGGL SIPHSTKRFP GYDSESKEFN AEVHRKHIMG QNVADYMRYL MEEDEDAYKK QFSQYIKNSV TPDMMEEMYK KA HAAIREN PVYEKKPKKE VKKKRWNRPK MSLAQKKDRV AQKKASFLRA QERAAES

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Macromolecule #45: ribosomal protein eL6

MacromoleculeName: ribosomal protein eL6 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 22.262428 KDa
SequenceString: PVGGDKNGGT RVVKLRKMPR YYPTEDVPRK LLSHGKKPFS QHVRKLRASI TPGTILIILT GRHRGKRVVF LKQLASGLLL VTGPLVLNR VPLRRTHQKF VIATSTKIDI SNVKIPKHLT DAYFKKKKLR KPRHQEGEIF DTEKEKYEIT EQRKIDQKAV D SQILPKIK ...String:
PVGGDKNGGT RVVKLRKMPR YYPTEDVPRK LLSHGKKPFS QHVRKLRASI TPGTILIILT GRHRGKRVVF LKQLASGLLL VTGPLVLNR VPLRRTHQKF VIATSTKIDI SNVKIPKHLT DAYFKKKKLR KPRHQEGEIF DTEKEKYEIT EQRKIDQKAV D SQILPKIK AIPQLQGYLR SVFALTNGIY PHKLVF

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Macromolecule #46: ribosomal protein uL30

MacromoleculeName: ribosomal protein uL30 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 27.733146 KDa
SequenceString: PETLKKKRRN FAELKIKRLR KKFAQKMLRK ARRKLIYEKA KHYHKEYRQM YRTEIRMARM ARKAGNFYVP AEPKLAFVIR IRGINGVSP KVRKVLQLLR LRQIFNGTFV KLNKASINML RIVEPYIAWG YPNLKSVNEL IYKRGYGKIN KKRIALTDNA L IARSLGKY ...String:
PETLKKKRRN FAELKIKRLR KKFAQKMLRK ARRKLIYEKA KHYHKEYRQM YRTEIRMARM ARKAGNFYVP AEPKLAFVIR IRGINGVSP KVRKVLQLLR LRQIFNGTFV KLNKASINML RIVEPYIAWG YPNLKSVNEL IYKRGYGKIN KKRIALTDNA L IARSLGKY GIICMEDLIH EIYTVGKRFK EANNFLWPFK LSSPRGGMKK KTTHFVEGGD AGNREDQINR LIRRMN

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Macromolecule #47: ribosomal protein eL8

MacromoleculeName: ribosomal protein eL8 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 26.724568 KDa
SequenceString: NPLFEKRPKN FGIGQDIQPK RDLTRFVKWP RYIRLQRQRA ILYKRLKVPP AINQFTQALD RQTATQLLKL AHKYRPETKQ EKKQRLLAR AEKKAAGKGD VPTKRPPVLR AGVNTVTTLV ENKKAQLVVI AHDVDPIELV VFLPALCRKM GVPYCIIKGK A RLGRLVHR ...String:
NPLFEKRPKN FGIGQDIQPK RDLTRFVKWP RYIRLQRQRA ILYKRLKVPP AINQFTQALD RQTATQLLKL AHKYRPETKQ EKKQRLLAR AEKKAAGKGD VPTKRPPVLR AGVNTVTTLV ENKKAQLVVI AHDVDPIELV VFLPALCRKM GVPYCIIKGK A RLGRLVHR KTCTTVAFTQ VNSEDKGALA KLVEAIRTNY NDRYDEIRRH WGGNVLGPKS VARIAKLEKA KAKELA

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Macromolecule #48: ribosomal protein uL6

MacromoleculeName: ribosomal protein uL6 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 21.770355 KDa
SequenceString:
MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QAD

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Macromolecule #49: ribosomal protein uL16

MacromoleculeName: ribosomal protein uL16 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.150484 KDa
SequenceString: RRPARCYRYC KNKPYPKSRF CRGVPDAKIR IFDLGRKKAK VDEFPLCGHM VSDEYEQLSS EALEAARICA NKYMVKSCGK DGFHIRVRL HPFHVIRINK MLSADRLQTG MRGAFGKPQG TVARVHIGQV IMSIRTKLQN KEHVIEALRR AKFKFPGRQK I HISKKWGF ...String:
RRPARCYRYC KNKPYPKSRF CRGVPDAKIR IFDLGRKKAK VDEFPLCGHM VSDEYEQLSS EALEAARICA NKYMVKSCGK DGFHIRVRL HPFHVIRINK MLSADRLQTG MRGAFGKPQG TVARVHIGQV IMSIRTKLQN KEHVIEALRR AKFKFPGRQK I HISKKWGF TKFNADEFED MVAEKRLIPD GCGVKYIPNR GPLDKWRALH

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Macromolecule #50: ribosomal protein uL5

MacromoleculeName: ribosomal protein uL5 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 19.270318 KDa
SequenceString:
ENPMRELRIR KLCLNICVGE SGDRLTRAAK VLEQLTGQTP VFSKARYTVR SFGIRRNEKI AVHCTVRGAK AEEILEKGLK VREYELRKN NFSDTGNFGF GIQEHIDLGI KYDPSIGIYG LDFYVVLGRP GFSIADKKRR TGCIGAKHRI SKEEAMRWFQ Q KYDGIILP G

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Macromolecule #51: ribosomal protein eL13

MacromoleculeName: ribosomal protein eL13 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 23.574697 KDa
SequenceString: APSRNGMVLK PHFHKDWQRR VATWFNQPAR KIRRRKARQA KARRIAPRPA SGPIRPIVRC PTVRYHTKVR AGRGFSLEEL RVAGIHKKV ARTIGISVDP RRRNKSTESL QANVQRLKEY RSKLILFPRK PSAPKKGDSS AEELKLATQL TGPVMPVRNV Y KKEKARVI ...String:
APSRNGMVLK PHFHKDWQRR VATWFNQPAR KIRRRKARQA KARRIAPRPA SGPIRPIVRC PTVRYHTKVR AGRGFSLEEL RVAGIHKKV ARTIGISVDP RRRNKSTESL QANVQRLKEY RSKLILFPRK PSAPKKGDSS AEELKLATQL TGPVMPVRNV Y KKEKARVI TEEEKNFKAF ASLRMARANA RLFGIRAKRA KEAAEQD

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Macromolecule #52: ribosomal protein eL14

MacromoleculeName: ribosomal protein eL14 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 16.220309 KDa
SequenceString:
VFRRFVEVGR VAYVSFGPHA GKLVAIVDVI DQNRALVDGP CTQVRRQAMP FKCMQLTDFI LKFPHSAHQK YVRQAWQKAD INTKWAATR WAKKIEARER KAKMTDFDRF KVMKAKKMRN RIIKNEVKKL QKAALLKASP

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Macromolecule #53: ribosomal protein eL15

MacromoleculeName: ribosomal protein eL15 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.076088 KDa
SequenceString: GAYKYIQELW RKKQSDVMRF LLRVRCWQYR QLSALHRAPR PTRPDKARRL GYKAKQGYVI YRIRVRRGGR KRPVPKGATY GKPVHHGVN QLKFARSLQS VAEERAGRHC GALRVLNSYW VGEDSTYKFF EVILIDPFHK AIRRNPDTQW ITKPVHKHRE M RGLTSAGR ...String:
GAYKYIQELW RKKQSDVMRF LLRVRCWQYR QLSALHRAPR PTRPDKARRL GYKAKQGYVI YRIRVRRGGR KRPVPKGATY GKPVHHGVN QLKFARSLQS VAEERAGRHC GALRVLNSYW VGEDSTYKFF EVILIDPFHK AIRRNPDTQW ITKPVHKHRE M RGLTSAGR KSRGLGKGHK FHHTIGGSRR AAWRRRNTLQ LHRYR

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Macromolecule #54: ribosomal protein uL13

MacromoleculeName: ribosomal protein uL13 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 22.820395 KDa
SequenceString: QVLVLDGRGH LLGRLAAIVA KQVLLGRKVV VVRCEGINIS GNFYRNKLKY LAFLRKRMNT NPSRGPYHFR APSRIFWRTV RGMLPHKTK RGQAALDRLK VFDGIPPPYD KKKRMVVPAA LKVVRLKPTR KFAYLGRLAH EVGWKYQAVT ATLEEKRKEK A KIHYRKKK ...String:
QVLVLDGRGH LLGRLAAIVA KQVLLGRKVV VVRCEGINIS GNFYRNKLKY LAFLRKRMNT NPSRGPYHFR APSRIFWRTV RGMLPHKTK RGQAALDRLK VFDGIPPPYD KKKRMVVPAA LKVVRLKPTR KFAYLGRLAH EVGWKYQAVT ATLEEKRKEK A KIHYRKKK QLMRLRKQAE KNVEKKIDKY TEVLKTH

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Macromolecule #55: ribosomal protein uL22

MacromoleculeName: ribosomal protein uL22 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 17.757627 KDa
SequenceString:
VRYSLDPENP TKSCKSRGSN LRVHFKNTRE TAQAIKGMHI RKATKYLKDV TLQKQCVPFR RYNGGVGRCA QAKQWGWTQG RWPKKSAEF LLHMLKNAES NAELKGLDVD SLVIEHIQVN KAPKMRRRTY RAHGRINPYM SSPCHIEMIL TEKE

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Macromolecule #56: ribosomal protein eL18

MacromoleculeName: ribosomal protein eL18 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 21.556482 KDa
SequenceString:
GVDIRHNKDR KVRRKEPKSQ DIYLRLLVKL YRFLARRTNS TFNQVVLKRL FMSRTNRPPL SLSRMIRKMK LPGRENKTAV VVGTITDDV RVQEVPKLKV CALRVTSRAR SRILRAGGKI LTFDQLALDS PKGCGTVLLS GPRKGREVYR HFGKAPGTPH S HTKPYVRS KGRKFERARG RRASRGYKN

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Macromolecule #57: ribosomal protein eL19

MacromoleculeName: ribosomal protein eL19 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 21.742146 KDa
SequenceString:
SMLRLQKRLA SSVLRCGKKK VWLDPNETNE IANANSRQQI RKLIKDGLII RKPVTVHSRA RCRKNTLARR KGRHMGIGKR KGTANARMP EKVTWMRRMR ILRRLLRRYR ESKKIDRHMY HSLYLKVKGN VFKNKRILME HIHKLKADKA RKKLLADQAE A RRSKTKEA RKRREERLQA KKE

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Macromolecule #58: ribosomal protein eL20

MacromoleculeName: ribosomal protein eL20 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 20.66134 KDa
SequenceString:
MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTF

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Macromolecule #59: ribosomal protein eL21

MacromoleculeName: ribosomal protein eL21 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 18.276518 KDa
SequenceString:
TNTKGKRRGT RYMFSRPFRK HGVVPLATYM RIYKKGDIVD IKGMGTVQKG MPHKCYHGKT GRVYNVTQHA VGIVVNKQVK GKILAKRIN VRIEHIKHSK SRDSFLKRVK ENDQKKKEAK EKGTWVQLKR QPAPPREAHF VRTNGKEPEL LEPIPYEF

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Macromolecule #60: ribosomal protein eL22

MacromoleculeName: ribosomal protein eL22 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 11.481248 KDa
SequenceString:
VLKFTLDCTH PVEDGIMDAA NFEQFLQERI KVNGKAGNLG GGVVTIERSK SKITVTSEVP FSKRYLKYLT KKYLKKNNLR DWLRVVANS KESYELRYFQ

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Macromolecule #61: ribosomal protein uL14

MacromoleculeName: ribosomal protein uL14 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 13.828295 KDa
SequenceString:
AKFRISLGLP VGAVINCADN TGAKNLYIIS VKGIKGRLNR LPAAGVGDMV MATVKKGKPE LRKKVHPAVV IRQRKSYRRK DGVFLYFED NAGVIVNNKG EMKGSAITGP VAKECADLWP RIASNAGSIA

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Macromolecule #62: ribosomal protein eL24

MacromoleculeName: ribosomal protein eL24 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 14.087417 KDa
SequenceString:
KVELCSFSGY KIYPGHGRRY ARTDGKVFQF LNAKCESAFL SKRNPRQINW TVLYRRKHKK GQSEEIQKKR TRRAVKFQRA ITGASLADI MAKRNQKPEV RKAQREQAIR AAKEAKKAKQ AS

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Macromolecule #63: ribosomal protein uL23

MacromoleculeName: ribosomal protein uL23 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 13.614023 KDa
SequenceString:
KIRTSPTFRR PKTLRLRRQP KYPRKSAPRR NKLDHYAIIK FPLTTESAMK KIEDNNTLVF IVDVKANKHQ IKQAVKKLYD IDVAKVNTL IRPDGEKKAY VRLAPDYDAL DVANKIGI

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Macromolecule #64: ribosomal protein uL24

MacromoleculeName: ribosomal protein uL24 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 15.161899 KDa
SequenceString:
MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQ

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Macromolecule #65: ribosomal protein eL27

MacromoleculeName: ribosomal protein eL27 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 15.647584 KDa
SequenceString:
KFMKPGKVVL VLAGRYSGRK AVIVKNIDDG TSDRPYSHAL VAGIDRYPRK VTAAMGKKKI AKRSKIKSFV KVYNYNHLMP TRYSVDIPL DKTVVNKDVF RDPALKRKAR REAKVKFEER YKTGKNKWFF QKLRF

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Macromolecule #66: ribosomal protein uL15

MacromoleculeName: ribosomal protein uL15 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 16.47334 KDa
SequenceString:
PSRLRKTRKL RGHVSHGHGR IGKHRKHPGG RGNAGGLHHH RINFDKYHPG YFGKVGMKHY HLKRNQSFCP TVNLDKLWTL VSEQTRVNA AKNKTGAAPI IDVVRSGYYK VLGKGKLPKQ PVIVKAKFFS RRAEEKIKSV GGACVLVA

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Macromolecule #67: ribosomal protein eL29

MacromoleculeName: ribosomal protein eL29 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 7.996388 KDa
SequenceString:
AKSKNHTTHN QSRKWHRNGI KKPRSQRYES LKGVDPKFLR NMRFAKKHNK KGLKKMQANN AKAMSARA

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Macromolecule #68: ribosomal protein eL30

MacromoleculeName: ribosomal protein eL30 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 11.500556 KDa
SequenceString:
TKKSLESINS RLQLVMKSGK YVLGYKQTLK MIRQGKAKLV ILANNCPALR KSEIEYYAML AKTGVHHYSG NNIELGTACG KYYRVCTLA IIDPGDSDII RSMP

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Macromolecule #69: ribosomal protein eL31

MacromoleculeName: ribosomal protein eL31 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 12.506581 KDa
SequenceString:
NEVVTREYTI NIHKRIHGVG FKKRAPRALK EIRKFAMKEM GTPDVRIDTR LNKAVWAKGI RNVPYRIRVR LSRKRNEDED SPNKLYTLV TYVPVTTFKN LQTVNVD

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Macromolecule #70: ribosomal protein eL32

MacromoleculeName: ribosomal protein eL32 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 15.179215 KDa
SequenceString:
AALRPLVKPK IVKKRTKKFI RHQSDRYVKI KRNWRKPRGI DNRVRRRFKG QILMPNIGYG SNKKTKHMLP SGFRKFLVHN VKELEVLLM CNKSYCAEIA HNVSSKNRKA IVERAAQLAI RVTNPNARLR

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Macromolecule #71: ribosomal protein eL33

MacromoleculeName: ribosomal protein eL33 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 12.433548 KDa
SequenceString:
SGRLWSKAIF AGYKRGLRNQ REHTALLKIE GVYARDETEF YLGKRCAYVY KAKNNTVTPG GKPNKTRVIW GKVTRAHGNS GMVRAKFRS NLPAKAIGHR IRVMLYPSRI

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Macromolecule #72: ribosomal protein eL34

MacromoleculeName: ribosomal protein eL34 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 12.994621 KDa
SequenceString:
VQRLTYRRRL SYNTASNKTR LSRTPGNRIV YLYTKKVGKA PKSACGVCPG RLRGVRAVRP KVLMRLSKTK KHVSRAYGGS MCAKCVRDR IKRAFLIEEQ KIVVKVLKAQ AQSQK

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Macromolecule #73: ribosomal protein uL29

MacromoleculeName: ribosomal protein uL29 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 14.462429 KDa
SequenceString:
AKIKARDLRG KKKEELLKQL DDLKVELSQL RVAKVTGGAA SKLSKIRVVR KSIARVLTVI NQTQKENLRK FYKGKKYKPL DLRPKKTRA MRRRLNKHEE NLKTKKQQRK ERLYPLRKYA VKA

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Macromolecule #74: ribosomal protein eL36

MacromoleculeName: ribosomal protein eL36 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 11.373708 KDa
SequenceString:
YPMAVGLNKG HKVTKNVSKP RHSRRRGRLT KHTKFVRDMI REVCGFAPYE RRAMELLKVS KDKRALKFIK KRVGTHIRAK RKREELSNV LAAMRKAA

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Macromolecule #75: ribosomal protein eL37

MacromoleculeName: ribosomal protein eL37 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 9.864596 KDa
SequenceString:
TKGTSSFGKR RNKTHTLCRR CGSKAYHLQK STCGKCGYPA KRKRKYNWSA KAKRRNTTGT GRMRHLKIVY RRFRHGFREG TTPK

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Macromolecule #76: ribosomal protein eL38

MacromoleculeName: ribosomal protein eL38 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 8.107752 KDa
SequenceString:
PRKIEEIKDF LLTARRKDAK SVKIKKNKDN VKFKVRCSRY LYTLVITDKE KAEKLKQSLP PGLAVKELK

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Macromolecule #77: ribosomal protein eL39

MacromoleculeName: ribosomal protein eL39 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 6.295562 KDa
SequenceString:
SSHKTFRIKR FLAKKQKQNR PIPQWIRMKT GNKIRYNSKR RHWRRTKLGL

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Macromolecule #78: ribosomal protein eL40

MacromoleculeName: ribosomal protein eL40 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 5.957237 KDa
SequenceString:
IEPSLRQLAQ KYNCDKMICR KCYARLHPRA VNCRKKKCGH TNNLRPKKKV

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Macromolecule #79: ribosomal protein eL41

MacromoleculeName: ribosomal protein eL41 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 3.473451 KDa
SequenceString:
MRAKWRKKRM RRLKRKRRKM RQRSK

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Macromolecule #80: ribosomal protein eL42

MacromoleculeName: ribosomal protein eL42 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 12.345776 KDa
SequenceString:
VNVPKTRRTF CKKCGKHQPH KVTQYKKGKD SLYAQGKRRY DRKQSGYGGQ TKPIFRKKAK TTKKIVLRLE CVEPNCRSKR MLAIKRCKH FELGGDKKRK GQVIQF

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Macromolecule #81: ribosomal protein eL43

MacromoleculeName: ribosomal protein eL43 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 10.168153 KDa
SequenceString:
AKRTKKVGIV GKYGTRYGAS LRKMVKKIEI SQHAKYTCSF CGKTKMKRRA VGIWHCGSCM KTVAGGAWTY NTTSAVTVKS AIRRLKELK DQ

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Macromolecule #82: ribosomal protein eL28

MacromoleculeName: ribosomal protein eL28 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 13.991366 KDa
SequenceString:
SAHLQWMVVR NCSSFLIKRN KQTYSTEPNN LKARNSFRYN GLIHRKTVGV EPAADGKGVV VVIKRRSGQR KPATSYVRTT INKNARATL SSIRHMIRKN KYRPDLRMAA IRRASAILRS QKP

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Macromolecule #83: ribosomal protein uL1

MacromoleculeName: ribosomal protein uL1 / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.879422 KDa
SequenceString: MSSKVSRDTL YEAVREVLHG NQRKRRKFLE TVELQISLKN YDPQKDKRFS GTVRLKSTPR PKFSVCVLGD QQHCDEAKAV DIPHMDIEA LKKLNKNKKL VKKLAKKYDA FLASESLIKQ IPRILGPGLN KAGKFPSLLT HNENMVAKVD EVKSTIKFQM K KVLCLAVA ...String:
MSSKVSRDTL YEAVREVLHG NQRKRRKFLE TVELQISLKN YDPQKDKRFS GTVRLKSTPR PKFSVCVLGD QQHCDEAKAV DIPHMDIEA LKKLNKNKKL VKKLAKKYDA FLASESLIKQ IPRILGPGLN KAGKFPSLLT HNENMVAKVD EVKSTIKFQM K KVLCLAVA VGHVKMTDDE LVYNIHLAVN FLVSLLKKNW QNVRALYIKS TMGKPQRLY

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Macromolecule #84: ribosomal protein uL11

MacromoleculeName: ribosomal protein uL11 / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 16.290794 KDa
SequenceString:
VYLRCTGGEV GATSALAPKI GPLGLSPKKV GDDIAKATGD WKGLRITVKL TIQNRQAQIE VVPSASALII KALKEPPRDR KKQKNIKHS GNITFDEIVN IARQMRHRSL ARELSGTIKE ILGTAQSVGC NVDGRHPHDI IDDINSGAVE CP

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Macromolecule #85: ribosomal protein uL10

MacromoleculeName: ribosomal protein uL10 / type: protein_or_peptide / ID: 85 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 22.21784 KDa
SequenceString: DRATWKSNYF LKIIQLLDDY PKCFIVGADN VGSKQMQQIR MSLRGKAVVL MGKNTMMRKA IRGHLENNPA LEKLLPHIRG NVGFVFTKE DLTEIRDMLR ANKVPAAARA GAIAPCEVTV PAQNTGLGPE KTSFFQALGI TTKISRGTIE ILSDVQLIKT G DKVGASEA ...String:
DRATWKSNYF LKIIQLLDDY PKCFIVGADN VGSKQMQQIR MSLRGKAVVL MGKNTMMRKA IRGHLENNPA LEKLLPHIRG NVGFVFTKE DLTEIRDMLR ANKVPAAARA GAIAPCEVTV PAQNTGLGPE KTSFFQALGI TTKISRGTIE ILSDVQLIKT G DKVGASEA TLLNMLNISP FSFGLVIQQV FDNGSIYNPE VLDI

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Macromolecule #86: eukaryotic elongation factor 2 (eEF2)

MacromoleculeName: eukaryotic elongation factor 2 (eEF2) / type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 95.056734 KDa
SequenceString: TVDQIRAIMD KKANIRNMSV IAHVDHGKST LTDSLVCKAG IIASARAGET RFTDTRKDEQ ERCITIKSTA ISLFYELSEN DLNFIKQSK DGAGFLINLI DSPGHVDFSS EVTAALRVTD GALVVVDCVS GVCVQTETVL RQAIAERIKP VLMMNKMDRA L LELQLEPE ...String:
TVDQIRAIMD KKANIRNMSV IAHVDHGKST LTDSLVCKAG IIASARAGET RFTDTRKDEQ ERCITIKSTA ISLFYELSEN DLNFIKQSK DGAGFLINLI DSPGHVDFSS EVTAALRVTD GALVVVDCVS GVCVQTETVL RQAIAERIKP VLMMNKMDRA L LELQLEPE ELYQTFQRIV ENVNVIISTY GEGESGPMGN IMIDPVLGTV GFGSGLHGWA FTLKQFAEMY VAKFAAKGEG QL GPAERAK KVEDMMKKLW GDRYFDPANG KFSKSATSPE GKKLPRTFCQ LILDPIFKVF DAIMNFKKEE TAKLIEKLDI KLD SEDKDK EGKPLLKAVM RRWLPAGDAL LQMITIHLPS PVTAQKYRCE LLYEGPPDDE AAMGIKSCDP KGPLMMYISK MVPT SDKGR FYAFGRVFSG LVSTGLKVRI MGPNYTPGKK EDLYLKPIQR TILMMGRYVE PIEDVPCGNI VGLVGVDQFL VKTGT ITTF EHAHNMRVMK FSVSPVVRVA VEAKNPADLP KLVEGLKRLA KSDPMVQCII EESGEHIIAG AGELHLEICL KDLEED HAC IPIKKSDPVV SYRETVSEES NVLCLSKSPN KHNRLYMKAR PFPDGLAEDI DKGEVSARQE LKQRARYLAE KYEWDVA EA RKIWCFGPDG TGPNILTDIT KGVQYLNEIK DSVVAGFQWA TKEGALCEEN MRGVRFDVHD VTLHADAI(DDE)R GGGQ IIPTA RRCLYASVLT AQPRLMEPIY LVEIQCPEQV VGGIYGVLNR KRGHVFEESQ VAGTPMFVVK AYLPVNESFG FTADL RSNT GGQAFPQCVF DHWQILPGDP FDNSSRPSQV VAETRKRKGL KEIPALDNFL DKL

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Macromolecule #87: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 87 / Number of copies: 327 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #88: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 88 / Number of copies: 5 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #89: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 89 / Number of copies: 1 / Formula: GNP
Molecular weightTheoretical: 522.196 Da
Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM / 5'-Guanylyl imidodiphosphate

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI POLARA 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average exposure time: 5.0 sec. / Average electron dose: 30.0 e/Å2
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 270000
Startup modelType of model: OTHER / Details: filtered empty 80S
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: SPIDER / Number images used: 44522

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-6gz5:
tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-3 (TI-POST-3)

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About Yorodumi

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News

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Feb 9, 2022. New format data for meta-information of EMDB entries

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