6GZ3

tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1)

This is a large structure.

Summary for 6GZ3

EMDB information0098
Descriptor28S ribosomal RNA, ap/P-site tRNA, mRNA, ... (89 entities in total)
Functional Keywordstranslocation rabbit ribosome, 80s, eef2, head swivel, rotation, ribosome
Biological sourceEnterobacteria phage SP6
More
Total number of polymer chains86
Total molecular weight3326031.43
Authors
Flis, J.,Holm, M.,Rundlet, E.J.,Loerke, J.,Hilal, T.,Dabrowski, M.,Buerger, J.,Mielke, T.,Blanchard, S.C.,Spahn, C.M.T.,Budkevich, T.V. (deposition date: 2018-07-03, release date: 2018-12-05, Last modification date: 2018-12-19)
Primary citation
Flis, J.,Holm, M.,Rundlet, E.J.,Loerke, J.,Hilal, T.,Dabrowski, M.,Burger, J.,Mielke, T.,Blanchard, S.C.,Spahn, C.M.T.,Budkevich, T.V.
tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis.
Cell Rep, 25:2676-2688.e7, 2018
PubMed: 30517857 (PDB entries with the same primary citation)
DOI: 10.1016/j.celrep.2018.11.040
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
?

Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone5 0.2% 0.9% 0.53MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 6gz3
no rotation
Molmil generated image of 6gz3
rotated about x axis by 90°
Molmil generated image of 6gz3
rotated about y axis by 90°
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