6GQ9

Solution structure of the hazel allergen Cor a 1.0401

Summary for 6GQ9

• Entry DOI: 10.2210/pdb6gq9/pdb
• NMR Information: BMRB: 34281
• Descriptor: Major allergen Cor a 1.0401 (1 entity in total)
• Functional Keywords: pr-10 allergen, hydrophobic pocket, bet v 1 homologue, allergen
• Biological source: Corylus avellana (European hazel)
• Total number of polymer chains: 1
• Total formula weight: 17606.07

Authors

Jacob, T.P.,Schweimer, K.,Seutter von Loetzen, C.,Woehrl, B.M. (deposition date: 2018-06-07, release date: 2019-06-19, Last modification date: 2024-05-15)

Primary citation

Jacob, T.,von Loetzen, C.S.,Reuter, A.,Lacher, U.,Schiller, D.,Schobert, R.,Mahler, V.,Vieths, S.,Rosch, P.,Schweimer, K.,Wohrl, B.M.
Identification of a natural ligand of the hazel allergen Cor a 1.
Sci Rep, 9:8714-8714, 2019

PubMed Abstract: Hazelnut is one of the most frequent causes of food allergy. The major hazel allergen in Northern Europe is Cor a 1, which is homologous to the major birch pollen allergen Bet v 1. Both allergens belong to the pathogenesis related class PR-10. We determined the solution structure of Cor a 1.0401 from hazelnut and identified a natural ligand of the protein. The structure reveals the protein fold characteristic for PR-10 family members, which consists of a seven-stranded antiparallel β-sheet, two short α-helices arranged in V-shape and a long C-terminal α-helix encompassing a hydrophobic pocket. However, despite the structural similarities between Cor a 1 and Bet v 1, they bind different ligands. We have shown previously that Bet v 1 binds to quercetin-3-O-sophoroside. Here, we isolated Cor a 1 from hazel pollen and identified the bound ligand, quercetin-3-O-(2"-O-β-D-glucopyranosyl)-β-D-galactopyranoside, by mass spectrometry and nuclear magnetic resonance spectroscopy (NMR). NMR experiments were performed to confirm binding. Remarkably, although it has been shown that PR-10 allergens show promiscuous binding behaviour in vitro, we can demonstrate that Cor a 1.0401 and Bet v 1.0101 exhibit highly selective binding for their specific ligand but not for the respective ligand of the other allergen.

PubMed: 31213622
DOI: 10.1038/s41598-019-44999-2

Experimental method

SOLUTION NMR