6GP1

Structure of mEos4b in the red long-lived dark state

6GP1 の概要

• エントリーDOI: 10.2210/pdb6gp1/pdb
• 関連するPDBエントリー: 6GOY 6GOZ 6GP0
• 分子名称: Green to red photoconvertible GFP-like protein EosFP (3 entities in total)
• 機能のキーワード: fluorescent protein
• 由来する生物種: Lobophyllia hemprichii (Lobed brain coral); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29566.30

構造登録者

De Zitter, E.,Adam, V.,Byrdin, M.,Van Meervelt, L.,Dedecker, P.,Bourgeois, D. (登録日: 2018-06-04, 公開日: 2019-05-22, 最終更新日: 2024-01-17)

主引用文献

De Zitter, E.,Thedie, D.,Monkemoller, V.,Hugelier, S.,Beaudouin, J.,Adam, V.,Byrdin, M.,Van Meervelt, L.,Dedecker, P.,Bourgeois, D.
Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM.
Nat.Methods, 16:707-710, 2019

PubMed Abstract: Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort.

PubMed: 31285624
DOI: 10.1038/s41592-019-0462-3

実験手法

X-RAY DIFFRACTION (1.504 Å)