6GOA

Structural basis for OXA-48 dimerization - R189A mutant

6GOA の概要

• エントリーDOI: 10.2210/pdb6goa/pdb
• 分子名称: Beta-lactamase, CHLORIDE ION (3 entities in total)
• 機能のキーワード: dimerization, oxa-48, oxacillinase, carbapenemase, beta-lactamase, antibiotic, hydrolase
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 225866.72

構造登録者

Lund, B.A.,Thomassen, A.M.,Leiros, H.K.S. (登録日: 2018-06-01, 公開日: 2018-07-18, 最終更新日: 2024-01-17)

主引用文献

Lund, B.A.,Thomassen, A.M.,Nesheim, B.H.B.,Carlsen, T.J.O.,Isaksson, J.,Christopeit, T.,Leiros, H.S.
The biological assembly of OXA-48 reveals a dimer interface with high charge complementarity and very high affinity.
FEBS J., 285:4214-4228, 2018

PubMed Abstract: Many class D β-lactamases form dimers in solution. The functional basis of the dimerization of OXA-48-like class D β-lactamases is not known, but in order to understand the structural requirements for dimerization of OXA-48, we have characterized the dimer interface. Size exclusion chromatography, small angle X-ray scattering (SAXS), and nuclear magnetic resonance (NMR) were used to confirm the oligomeric state of OXA-48 in solution. X-ray crystallographic structures were used to elucidate the key interactions of dimerization. In silico residue scanning combined with site-directed mutagenesis was used to probe hot spots of dimerization. The affinity of dimerization was quantified using microscale thermophoresis, and the overall thermostability was investigated using differential scanning calorimetry. OXA-48 was consistently found to be a dimer in solution regardless of the method used, and the biological assembly found from the SAXS envelope is consistent with the dimer identified from the crystal structures. The buried chloride that interacts with Arg206 and Arg206' at the dimer interface was found to enhance the thermal stability by > 4 °C and crystal structures and mutations (R189A, R189A/R206A) identified several additional important ionic interactions. The affinity for OXA-48 R206A dimerization was in the picomolar range, thus revealing very high dimer affinity. In summary, OXA-48 has a very stable dimer interface, facilitated by noncovalent and predominantly charged interactions, which is stronger than the dimer interfaces previously described for other class D β-lactamases. PDB CODES: The oxacillinase-48 (OXA-48) R206A structure has PDB ID: 5OFT and OXA-48 R189A has PDB ID: 6GOA.

PubMed: 30153368
DOI: 10.1111/febs.14643

実験手法

X-RAY DIFFRACTION (2.55 Å)