6GL4

Structure of GluA2o ligand-binding domain (S1S2J) in complex with glutamate and sodium bromide at 1.95 A resolution

6GL4 の概要

• エントリーDOI: 10.2210/pdb6gl4/pdb
• 分子名称: Glutamate receptor 2,Glutamate receptor 2, GLUTAMIC ACID, BROMIDE ION, ... (7 entities in total)
• 機能のキーワード: ionotropic glutamate receptor, glua2o ligand-binding domain, agonist, membrane protein
• 由来する生物種: Rattus norvegicus (Rat); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59528.26

構造登録者

Venskutonyte, R.,Frydenvang, K.,Kastrup, J.S. (登録日: 2018-05-22, 公開日: 2019-05-15, 最終更新日: 2024-10-16)

主引用文献

Dawe, G.B.,Kadir, M.F.,Venskutonyte, R.,Perozzo, A.M.,Yan, Y.,Alexander, R.P.D.,Navarrete, C.,Santander, E.A.,Arsenault, M.,Fuentes, C.,Aurousseau, M.R.P.,Frydenvang, K.,Barrera, N.P.,Kastrup, J.S.,Edwardson, J.M.,Bowie, D.
Nanoscale Mobility of the Apo State and TARP Stoichiometry Dictate the Gating Behavior of Alternatively Spliced AMPA Receptors.
Neuron, 102:976-, 2019

PubMed Abstract: Neurotransmitter-gated ion channels are allosteric proteins that switch on and off in response to agonist binding. Most studies have focused on the agonist-bound, activated channel while assigning a lesser role to the apo or resting state. Here, we show that nanoscale mobility of resting α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type ionotropic glutamate receptors (AMPA receptors) predetermines responsiveness to neurotransmitter, allosteric anions and TARP auxiliary subunits. Mobility at rest is regulated by alternative splicing of the flip/flop cassette of the ligand-binding domain, which controls motions in the distant AMPA receptor N-terminal domain (NTD). Flip variants promote moderate NTD movement, which establishes slower channel desensitization and robust regulation by anions and auxiliary subunits. In contrast, greater NTD mobility imparted by the flop cassette acts as a master switch to override allosteric regulation. In AMPA receptor heteromers, TARP stoichiometry further modifies these actions of the flip/flop cassette generating two functionally distinct classes of partially and fully TARPed receptors typical of cerebellar stellate and Purkinje cells.

PubMed: 31053408
DOI: 10.1016/j.neuron.2019.03.046

実験手法

X-RAY DIFFRACTION (1.948 Å)