6GJS

Human NBD1 of CFTR in complex with nanobodies D12 and T4

6GJS の概要

• エントリーDOI: 10.2210/pdb6gjs/pdb
• 分子名称: Cystic fibrosis transmembrane conductance regulator, Nanobody D12, Nanobody T4, ... (6 entities in total)
• 機能のキーワード: cystic fibrosis, cftr, nanobodies, thermal stabilization, conformational dynamics, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 57843.24

構造登録者

Sigoillot, M.,Overtus, M.,Grodecka, M.,Scholl, D.,Garcia-Pino, A.,Laeremans, T.,He, L.,Pardon, E.,Hildebrandt, E.,Urbatsch, I.,Steyaert, J.,Riordan, J.R.,Govaerts, C. (登録日: 2018-05-16, 公開日: 2019-06-26, 最終更新日: 2024-11-13)

主引用文献

Sigoillot, M.,Overtus, M.,Grodecka, M.,Scholl, D.,Garcia-Pino, A.,Laeremans, T.,He, L.,Pardon, E.,Hildebrandt, E.,Urbatsch, I.,Steyaert, J.,Riordan, J.R.,Govaerts, C.
Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.
Nat Commun, 10:2636-2636, 2019

PubMed Abstract: The leading cause of cystic fibrosis (CF) is the deletion of phenylalanine 508 (F508del) in the first nucleotide-binding domain (NBD1) of the cystic fibrosis transmembrane conductance regulator (CFTR). The mutation affects the thermodynamic stability of the domain and the integrity of the interface between NBD1 and the transmembrane domain leading to its clearance by the quality control system. Here, we develop nanobodies targeting NBD1 of human CFTR and demonstrate their ability to stabilize both isolated NBD1 and full-length protein. Crystal structures of NBD1-nanobody complexes provide an atomic description of the epitopes and reveal the molecular basis for stabilization. Furthermore, our data uncover a conformation of CFTR, involving detachment of NBD1 from the transmembrane domain, which contrast with the compact assembly observed in cryo-EM structures. This unexpected interface rearrangement is likely to have major relevance for CF pathogenesis but also for the normal function of CFTR and other ABC proteins.

PubMed: 31201318
DOI: 10.1038/s41467-019-10714-y

実験手法

X-RAY DIFFRACTION (1.95 Å)