6GH8

Crystal structure of GP1 domain of Lujo virus in complex with the first CUB domain of neuropilin-2

6GH8 の概要

• エントリーDOI: 10.2210/pdb6gh8/pdb
• 分子名称: Glycoprotein, Neuropilin-2, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: viral glycoprotein, receptor recognition, viral protein
• 由来する生物種: Lujo mammarenavirus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 62616.57

構造登録者

Cohen-Dvashi, H.,Kilimnik, I.,Diskin, R. (登録日: 2018-05-06, 公開日: 2018-07-25, 最終更新日: 2024-11-06)

主引用文献

Cohen-Dvashi, H.,Kilimnik, I.,Diskin, R.
Structural basis for receptor recognition by Lujo virus.
Nat Microbiol, 3:1153-1160, 2018

PubMed Abstract: Lujo virus (LUJV) has emerged as a highly fatal human pathogen. Despite its membership among the Arenaviridae, LUJV does not classify with the known Old and New World groups of that viral family. Likewise, LUJV was recently found to use neuropilin-2 (NRP2) as a cellular receptor instead of the canonical receptors used by Old World and New World arenaviruses. The emergence of a deadly pathogen into human populations using an unprecedented entry route raises many questions regarding the mechanism of cell recognition. To provide the basis for combating LUJV in particular, and to increase our general understanding of the molecular changes that accompany an evolutionary switch to a new receptor for arenaviruses, we used X-ray crystallography to reveal how the GP1 receptor-binding domain of LUJV (LUJV) recognizes NRP2. Structural data show that LUJV is more similar to Old World than to New World arenaviruses. Structural analysis supported by experimental validation further suggests that NRP2 recognition is metal-ion dependent and that the complete NRP2 binding site is formed in the context of the trimeric spike. Taken together, our data provide the mechanism for the cell attachment step of LUJV and present indispensable information for combating this phatogen.

PubMed: 30150732
DOI: 10.1038/s41564-018-0224-5

実験手法

X-RAY DIFFRACTION (2.44 Å)