6GD8

Cytochrome c in complex with Sulfonato-calix[8]arene, P31 form

6GD8 の概要

• エントリーDOI: 10.2210/pdb6gd8/pdb
• 関連するPDBエントリー: 6GD6 6GD7 6GD9 6GDA
• 分子名称: Cytochrome c iso-1, HEME C, sulfonato-calix[8]arene, ... (4 entities in total)
• 機能のキーワード: calixarene, scaffold, supramolecular, assembly, oxidoreductase
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 56599.02

構造登録者

Rennie, M.L.,Fox, G.C.,Crowley, P.B. (登録日: 2018-04-23, 公開日: 2018-08-29, 最終更新日: 2024-01-17)

主引用文献

Rennie, M.L.,Fox, G.C.,Perez, J.,Crowley, P.B.
Auto-regulated Protein Assembly on a Supramolecular Scaffold.
Angew. Chem. Int. Ed. Engl., 57:13764-13769, 2018

PubMed Abstract: Controlled protein assembly provides a means to regulate function. Supramolecular building blocks, including rigid macrocycles, are versatile triggers of protein assembly. Now it is shown that sulfonato-calix[8]arene (sclx ) mediates the formation of cytochrome c tetramers in solution. This tetramer spontaneously disassembles at ≥2 equivalents of sclx , providing a remarkable example of auto-regulation. Using X-ray crystallography the sclx binding sites on cytochrome c were characterized. Crystal structures at different protein-ligand ratios reveal varying degrees (up to 35 %) of protein surface coverage by the flexible calixarene and suggest a mechanism for oligomer disassembly. The solution structure of the oligomer was characterized by small-angle X-ray scattering. Overall, the data indicate calixarene-controlled protein assembly and disassembly without the requirement for a competitive inhibitor, and point to protein encapsulation by a flexible macrocycle.

PubMed: 30109907
DOI: 10.1002/anie.201807490

実験手法

X-RAY DIFFRACTION (2.5 Å)