6GCS

Cryo-EM structure of respiratory complex I from Yarrowia lipolytica

6GCS の概要

• エントリーDOI: 10.2210/pdb6gcs/pdb
• EMDBエントリー: 4384
• 分子名称: 75-KDA PROTEIN (NUAM), NUJM SUBUNIT, PSST SUBUNIT (NUKM), ... (50 entities in total)
• 機能のキーワード: complex i, nadh dehydrogenase, mitochondrion proton pumping, ubiquinone, oxidoreductase
• 由来する生物種: Yarrowia lipolytica; 詳細
• タンパク質・核酸の鎖数: 42
• 化学式量合計: 935297.12

構造登録者

Parey, K.,Vonck, J. (登録日: 2018-04-19, 公開日: 2018-10-10, 最終更新日: 2024-10-09)

主引用文献

Parey, K.,Brandt, U.,Xie, H.,Mills, D.J.,Siegmund, K.,Vonck, J.,Kuehlbrandt, W.,Zickermann, V.
Cryo-EM structure of respiratory complex I at work.
Elife, 7:-, 2018

PubMed Abstract: Mitochondrial complex I has a key role in cellular energy metabolism, generating a major portion of the proton motive force that drives aerobic ATP synthesis. The hydrophilic arm of the L-shaped ~1 MDa membrane protein complex transfers electrons from NADH to ubiquinone, providing the energy to drive proton pumping at distant sites in the membrane arm. The critical steps of energy conversion are associated with the redox chemistry of ubiquinone. We report the cryo-EM structure of complete mitochondrial complex I from the aerobic yeast both in the deactive form and after capturing the enzyme during steady-state activity. The site of ubiquinone binding observed during turnover supports a two-state stabilization change mechanism for complex I.

PubMed: 30277212
DOI: 10.7554/eLife.39213

実験手法

ELECTRON MICROSCOPY (4.32 Å)