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- EMDB-4384: Cryo-EM structure of respiratory complex I from Yarrowia lipolytica -

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Basic information

Entry
Database: EMDB / ID: EMD-4384
TitleCryo-EM structure of respiratory complex I from Yarrowia lipolytica
Map data
Sample
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase
    • Protein or peptide: x 42 types
  • Ligand: x 8 types
Function / homology
Function and homology information


lipoate biosynthetic process / NADH dehydrogenase / TIM23 mitochondrial import inner membrane translocase complex / mitochondrial iron-sulfur cluster assembly complex / protein import into mitochondrial matrix / ubiquinone-6 biosynthetic process / NADH dehydrogenase activity / respiratory chain complex I / ubiquinone binding / oxidoreductase activity, acting on NAD(P)H ...lipoate biosynthetic process / NADH dehydrogenase / TIM23 mitochondrial import inner membrane translocase complex / mitochondrial iron-sulfur cluster assembly complex / protein import into mitochondrial matrix / ubiquinone-6 biosynthetic process / NADH dehydrogenase activity / respiratory chain complex I / ubiquinone binding / oxidoreductase activity, acting on NAD(P)H / acyl binding / electron transport coupled proton transport / iron-sulfur cluster assembly / acyl carrier activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport chain / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / respirasome / aerobic respiration / respiratory electron transport chain / mitochondrial membrane / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / fatty acid biosynthetic process / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / response to oxidative stress / mitochondrial inner membrane / oxidoreductase activity / protein-containing complex binding / mitochondrion / membrane / metal ion binding
Similarity search - Function
NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 ...NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / Soluble ligand binding domain / Zinc finger, CHCC-type / Zinc-finger domain / SLBB domain / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NDUFA6, LYR domain / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NDUFB9, LYR domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NAD(P)H-quinone oxidoreductase subunit D/H / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH-quinone oxidoreductase, subunit D / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH dehydrogenase, subunit C / NADH ubiquinone oxidoreductase, F subunit / Complex 1 LYR protein domain / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature. / NADH-quinone oxidoreductase, chain M/4 / Complex 1 protein (LYR family) / 2Fe-2S iron-sulfur cluster binding domain / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH:ubiquinone oxidoreductase, 30kDa subunit / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily / Respiratory-chain NADH dehydrogenase, 30 Kd subunit / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / NADH:ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit 1, conserved site / Respiratory-chain NADH dehydrogenase subunit 1 signature 1. / Respiratory-chain NADH dehydrogenase subunit 1 signature 2. / NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H / NADH dehydrogenase / CHCH / NuoE domain / CHCH domain / NADH:ubiquinone oxidoreductase / NADH-quinone oxidoreductase subunit E-like / Thioredoxin-like [2Fe-2S] ferredoxin / NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit / Proton-conducting membrane transporter / NADH-quinone oxidoreductase subunit E, N-terminal / NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding / His(Cys)3-ligated-type [4Fe-4S] domain profile. / NADH:ubiquinone oxidoreductase, 51kDa subunit, conserved site / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. / NADH-ubiquinone oxidoreductase 51kDa subunit, FMN-binding domain / NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain / NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain superfamily
Similarity search - Domain/homology
Complex 1 LYR protein domain-containing protein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / Acyl carrier protein / Uncharacterized protein / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit-domain-containing protein / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / Thioredoxin-like protein ...Complex 1 LYR protein domain-containing protein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / Acyl carrier protein / Uncharacterized protein / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit-domain-containing protein / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / Thioredoxin-like protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / Acyl carrier protein / Zinc finger CHCC-type domain-containing protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / YALI0D10274p / YALI0E23749p / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / YALI0A17946p / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / YALI0E28424p / YALI0E23089p / YALI0E11891p / Acyl carrier protein / YALI0D24585p / YALI0D19030p / Acyl carrier protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / YALI0C03201p / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit N7BM / NADH-ubiquinone oxidoreductase / YALI0A01419p / NUVM protein / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 6 / Subunit NUKM of protein NADH:Ubiquinone oxidoreductase / Subunit NUIM of protein NADH:Ubiquinone oxidoreductase / Subunit NUHM of protein NADH:Ubiquinone oxidoreductase / NUGM protein / NUCM protein / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NUAM protein
Similarity search - Component
Biological speciesYarrowia lipolytica (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.32 Å
AuthorsParey K / Vonck J
Funding support Germany, 1 items
OrganizationGrant numberCountry
German Research FoundationEXC 115 Germany
CitationJournal: Elife / Year: 2018
Title: Cryo-EM structure of respiratory complex I at work.
Authors: Kristian Parey / Ulrich Brandt / Hao Xie / Deryck J Mills / Karin Siegmund / Janet Vonck / Werner Kühlbrandt / Volker Zickermann /
Abstract: Mitochondrial complex I has a key role in cellular energy metabolism, generating a major portion of the proton motive force that drives aerobic ATP synthesis. The hydrophilic arm of the L-shaped ~1 ...Mitochondrial complex I has a key role in cellular energy metabolism, generating a major portion of the proton motive force that drives aerobic ATP synthesis. The hydrophilic arm of the L-shaped ~1 MDa membrane protein complex transfers electrons from NADH to ubiquinone, providing the energy to drive proton pumping at distant sites in the membrane arm. The critical steps of energy conversion are associated with the redox chemistry of ubiquinone. We report the cryo-EM structure of complete mitochondrial complex I from the aerobic yeast both in the deactive form and after capturing the enzyme during steady-state activity. The site of ubiquinone binding observed during turnover supports a two-state stabilization change mechanism for complex I.
History
DepositionApr 19, 2018-
Header (metadata) releaseJul 18, 2018-
Map releaseOct 10, 2018-
UpdateJun 2, 2021-
Current statusJun 2, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.045
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.045
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6gcs
  • Surface level: 0.045
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4384.png

Downloads & links

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Map

FileDownload / File: emd_4384.map.gz / Format: CCP4 / Size: 361.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.09 Å
Density
Contour LevelBy AUTHOR: 0.045 / Movie #1: 0.045
Minimum - Maximum-0.0568656 - 0.25599003
Average (Standard dev.)0.00036767506 (±0.004647704)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions456456456
Spacing456456456
CellA=B=C: 497.04 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.091.091.09
M x/y/z456456456
origin x/y/z0.0000.0000.000
length x/y/z497.040497.040497.040
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS456456456
D min/max/mean-0.0570.2560.000

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Supplemental data

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Sample components

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Entire : Mitochondrial NADH:ubiquinone oxidoreductase

EntireName: Mitochondrial NADH:ubiquinone oxidoreductase
Components
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase
    • Protein or peptide: 75-KDA PROTEIN (NUAM)
    • Protein or peptide: 51-KDA PROTEIN (NUBM)
    • Protein or peptide: 49-KDA PROTEIN (NUCM)
    • Protein or peptide: NIMM SUBUNIT
    • Protein or peptide: NUEM SUBUNIT
    • Protein or peptide: NUFM SUBUNIT
    • Protein or peptide: 30-KDA PROTEIN (NUGM)
    • Protein or peptide: 24-KDA SUBUNIT (NUHM)
    • Protein or peptide: TYKY SUBUNIT (NUIM)
    • Protein or peptide: NUJM SUBUNIT
    • Protein or peptide: PSST SUBUNIT (NUKM)
    • Protein or peptide: ND4L SUBUNIT (NULM)
    • Protein or peptide: NUMM SUBUNIT
    • Protein or peptide: ACPM1 SUBUNIT
    • Protein or peptide: NB4M SUBUNIT
    • Protein or peptide: ACPM2 SUBUNIT
    • Protein or peptide: NI2M SUBUNIT
    • Protein or peptide: NESM SUBUNIT
    • Protein or peptide: NUPM SUBUNIT
    • Protein or peptide: NB6M SUBUNIT
    • Protein or peptide: NUXM SUBUNIT
    • Protein or peptide: NUYM SUBUNIT
    • Protein or peptide: NUZM SUBUNIT
    • Protein or peptide: NIAM SUBUNIT
    • Protein or peptide: NEBM SUBUNIT
    • Protein or peptide: NB2M SUBUNIT
    • Protein or peptide: NIDM SUBUNIT
    • Protein or peptide: NUUM SUBUNIT
    • Protein or peptide: NI8M SUBUNIT
    • Protein or peptide: NI9M SUBUNIT
    • Protein or peptide: N7BM SUBUNIT
    • Protein or peptide: UNKNOWN SUBUNIT
    • Protein or peptide: NB5M SUBUNIT
    • Protein or peptide: NUNM SUBUNIT
    • Protein or peptide: ND1 SUBUNIT (NU1M)
    • Protein or peptide: ND2 SUBUNIT (NU2M)
    • Protein or peptide: ND3 SUBUNIT (NU3M)
    • Protein or peptide: ND4 SUBUNIT (NU4M)
    • Protein or peptide: ND5 SUBUNIT (NU5M)
    • Protein or peptide: ND6 SUBUNIT (NU6M)
    • Protein or peptide: NB8M SUBUNIT
    • Protein or peptide: NIPM SUBUNIT
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: FLAVIN MONONUCLEOTIDE
  • Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
  • Ligand: ZINC ION
  • Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
  • Ligand: CARDIOLIPIN
  • Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE

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Supramolecule #1: Mitochondrial NADH:ubiquinone oxidoreductase

SupramoleculeName: Mitochondrial NADH:ubiquinone oxidoreductase / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#42
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 963.7 kDa/nm

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Macromolecule #1: 75-KDA PROTEIN (NUAM)

MacromoleculeName: 75-KDA PROTEIN (NUAM) / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 79.088078 KDa
SequenceString: MLSRNLSKFA RAGLIRPATT STHTRLFSVS ARRLAEIELT IDGHKVSIEA GSALIQACEK AGVTVPRYCY HDKLAIAGNC RMCLVDVER APKPVASCAY PVAPGMVVRT DTERVKQARE NVMEMMLQNH PLDCPVCDQG GECDLQDQSM RYGRDRGRFT E ITGKRSTE ...String:
MLSRNLSKFA RAGLIRPATT STHTRLFSVS ARRLAEIELT IDGHKVSIEA GSALIQACEK AGVTVPRYCY HDKLAIAGNC RMCLVDVER APKPVASCAY PVAPGMVVRT DTERVKQARE NVMEMMLQNH PLDCPVCDQG GECDLQDQSM RYGRDRGRFT E ITGKRSTE DKNIGPLVKT SMNRCIHCTR CVRFANDIAG APELGSSGRG NDMQIGTYLE KNLNTELSGN VIDLCPVGAL TN KPYAFRA RPWELKKTES IDVMDAVGSN IRIDSKGVEV MRVIPRVHED VNEEWINDKS RFACDGLKTQ RLTTPLIRVG DKF VNATWD DALSTIAKAY QQKAPKGDEF KAVAGALVEV ESMVALKDMT NALGSENTTT DTPNGNSAPA HGITFRSNYL FNSS IAGIE DADAILLVGT NPRREAAVMN ARIRKAWLRQ ELEIASVGPT LDATFDVAEL GNTHADLEKA LSGEFGEVLK NAKNP LIIV GSGITDREDA GAFFNTIGKF VESTPSVLNE NWNGYNVLQR SASRAGAYDI GFTPSDEASK TTPKMVWLLG ADEVAA SDI PADAFVVYQG HNGDVGAQFA DVVLPGAAYT EKAGTYVNTE GRSQISRAAT GPPGGAREDW KILRAVSEYL GVALPYE DA YEVRDRLAEI SPSLVRYDLV EPTVFGDVAV QHSLVGPNGS VTPSSAPLTE TIENFYMTDS ISRSSPTMAK SSIAFNKD N KKNQAFA

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Macromolecule #2: 51-KDA PROTEIN (NUBM)

MacromoleculeName: 51-KDA PROTEIN (NUBM) / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 53.829508 KDa
SequenceString: MLRTTLHKRG LARLSRGFAT TQDATPKARQ YGGLKDQDRI FQNLYDNYGW DLASARKQGD WYKTKELILK GDTWIIDEIK KSGLRGRGG AGFPSGLKWS FMNPPGWEKN EGPRYLVVNA DEGEPGTCKD REIMRKDPHK LVEGCLLAGR AMNATAAYIY I RGEFYNEA ...String:
MLRTTLHKRG LARLSRGFAT TQDATPKARQ YGGLKDQDRI FQNLYDNYGW DLASARKQGD WYKTKELILK GDTWIIDEIK KSGLRGRGG AGFPSGLKWS FMNPPGWEKN EGPRYLVVNA DEGEPGTCKD REIMRKDPHK LVEGCLLAGR AMNATAAYIY I RGEFYNEA AVLQTAINEA YAAGLIGKDA CGSGYDFDVY IHRGMGAYVC GEETSLIESL EGKAGKPRLK PPFPAGVGLF GR PSTVTNV ETVAVAPTIL RRGGDWFASF GRERNSGTKL FCISGNVNEP CTVEEEMSIP LRELLEKHCG GIKGGWDNLL GVI PGGCSV PILPKNICED VLMDFDALKD VQSGLGTAAV IVINKQQDVI RAIQRFAAFY KHESCGQCTP CREGTTWLLK AMDR FRTGQ AKEREIDMLY ELTKDIEGHT ICALGDAAAW PIQGLIRNFR PEMETRMKKF HDEVGAVSVG GWMKDARVEK GKVVG APLP GVHH

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Macromolecule #3: 49-KDA PROTEIN (NUCM)

MacromoleculeName: 49-KDA PROTEIN (NUCM) / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 52.494906 KDa
SequenceString: MLRSAAARAV RAVRPRLSAR YMATTALPQD PIPSGALGQK VPHVDESHQD LLFRTSHMVE DLETYDEDSP INTSDANTRI RAFTINFGP QHPAAHGVLR LILELSGEEI IRSDPHVGLL HRGTEKLIEY KTYMQALPYF DRLDYVSMMT NEQVFSLAVE K LLNVEVPL ...String:
MLRSAAARAV RAVRPRLSAR YMATTALPQD PIPSGALGQK VPHVDESHQD LLFRTSHMVE DLETYDEDSP INTSDANTRI RAFTINFGP QHPAAHGVLR LILELSGEEI IRSDPHVGLL HRGTEKLIEY KTYMQALPYF DRLDYVSMMT NEQVFSLAVE K LLNVEVPL RGKYIRTMFG EITRVLNHLM SVCSHAMDVG ALTPFLWGFE EREKLMEFYE RVSGARLHAA YVRPGGVSQD LP AGLLDDI YMWATQFGDR LDEIEELLTD NRIWKLRTVN IGTVTAQDAL NLGLSGPMLR GSGIPFDIRK NAPYDAYDKV DFD VPVGMN GDCYDRYLIR MAEFRQSLRI IEQCCNDMPA GAVKVEDFKI NSPPRNLMKE DMEALIHHFL LYTKGYSVPP GETY TAIEA PKGEMGVYVV SDGSERPYKC KIRAPGFAHL GAFDHIARGH FLPDAVAIIG TMDLVFGEVD R

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Macromolecule #4: NIMM SUBUNIT

MacromoleculeName: NIMM SUBUNIT / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 9.806238 KDa
SequenceString:
MAIPFEALLP YGIIFGLLTA GGGAMQVLHV YRNGGVRDRF AIDQWDSQMM ERDLRLNGGQ GRKQVDQATA PEAFKHNHVW KSERPLI

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Macromolecule #5: NUEM SUBUNIT

MacromoleculeName: NUEM SUBUNIT / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 42.765738 KDa
SequenceString: MLRTTRAVKP LKAITTSVRF MNSFENLAQD VNITRSGKTL IAKGTGGRSS RTGYTATVFG ANGFLGSYLT AKLAKHGTTV VVPYREEMA KRHLKVTGDL GVVNFLEMDL RNLESIDEAV RHSDIVVNLI GREYETKNFN YYDVHVEGAR RIAEAVKKHN I ARYIHVSA ...String:
MLRTTRAVKP LKAITTSVRF MNSFENLAQD VNITRSGKTL IAKGTGGRSS RTGYTATVFG ANGFLGSYLT AKLAKHGTTV VVPYREEMA KRHLKVTGDL GVVNFLEMDL RNLESIDEAV RHSDIVVNLI GREYETKNFN YYDVHVEGAR RIAEAVKKHN I ARYIHVSA FNAEIDSPSE FNHTKGLGEQ VTKDIVPWAT IVRPAPMFGR EDKWFLDRMA RSPCLVSANK FQETSNPVHV ID VAAALER ICFDDSTVAQ TFELYGPQKF TQKQIIDMVS ETLRKEVRHI ELPKALYQAY TKATQAIWWP TYSPDQVERQ FLS QKIDPS AKTFNDLDLT PMELPDLMFK LIRPYRVNTF QHDVSQLENK EKTFVHILD

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Macromolecule #6: NUFM SUBUNIT

MacromoleculeName: NUFM SUBUNIT / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 16.65792 KDa
SequenceString:
MRYTQLLRNV SKGVQKSGQS VLVQMREGTP IGLTGIYQHP NPRPALIALY EATLKELQDK HPKDSVYRQS IENLTAHRKQ IVEDNEVSE VIENKIGAGL IEEVVIQAHE ELELAKKMSE WKPWEELEEK PLEDQWVYFN KKGVE

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Macromolecule #7: 30-KDA PROTEIN (NUGM)

MacromoleculeName: 30-KDA PROTEIN (NUGM) / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 32.389568 KDa
SequenceString: MLSRFARIGS MGIRPVAAAR ATFVTSARAA QAAPSWENIK DIRLDPKVHV DEVYEPIVNP ADRYLQHVSD LHQYAKYIMA ALPKYIQGF SVWKDELTLH VAPSAVIPVT TFLRDNTSTQ YKSIIDITAV DYPSRENRFE VVYNFLSVRH NSRIRLKTYA T EVTPVPSI ...String:
MLSRFARIGS MGIRPVAAAR ATFVTSARAA QAAPSWENIK DIRLDPKVHV DEVYEPIVNP ADRYLQHVSD LHQYAKYIMA ALPKYIQGF SVWKDELTLH VAPSAVIPVT TFLRDNTSTQ YKSIIDITAV DYPSRENRFE VVYNFLSVRH NSRIRLKTYA T EVTPVPSI TCLYEGANWF EREAYDMYGV FFEGHPDLRR IMTDYGFEGH PLRKDFPLTG YTEVRWDEEK RRVVYEPLEL TQ AFRNFSA GSTAWEPVGP GRDDRPDSFK LPTPKPEEKE GDKK

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Macromolecule #8: 24-KDA SUBUNIT (NUHM)

MacromoleculeName: 24-KDA SUBUNIT (NUHM) / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 27.247402 KDa
SequenceString: MLRLIRPRLA ALARPTTRAP QALNARTHIV SVHRNTENNN PSIPFEFSPE NMKRAEEVIA KYPPQYKKAA VMPLLDIGQR QLGYTSISV MNYVAKLLEM PPMRVYEVAT FYTMYNRTPM GRYHLQICTT TPCQLCGSDG IMEAVQNTLN IKPGETTKDN L FTLSEVEC ...String:
MLRLIRPRLA ALARPTTRAP QALNARTHIV SVHRNTENNN PSIPFEFSPE NMKRAEEVIA KYPPQYKKAA VMPLLDIGQR QLGYTSISV MNYVAKLLEM PPMRVYEVAT FYTMYNRTPM GRYHLQICTT TPCQLCGSDG IMEAVQNTLN IKPGETTKDN L FTLSEVEC LGACVNAPMM AINDDYYEDL TPEGTVKLLE DCKAGKMPTP GPENHVRRDC EPASGQKVLL SKEPHNVADF LQ EGI

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Macromolecule #9: TYKY SUBUNIT (NUIM)

MacromoleculeName: TYKY SUBUNIT (NUIM) / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 25.682305 KDa
SequenceString: MLSLVRPAVT RSILRGAPGS MRLLSSTARL HAPATDSAIN IYAGGSAAAA PPAGFRIHRP ATWEESEEGA LSKATKYFLL AEMFRGLYV VLEQFFRAPY TIYYPFEKGP VSPRFRGEHA LRRYPSGEER CIACKLCEAI CPALAITIDA EERIDGSRRT T KYDIDMTK ...String:
MLSLVRPAVT RSILRGAPGS MRLLSSTARL HAPATDSAIN IYAGGSAAAA PPAGFRIHRP ATWEESEEGA LSKATKYFLL AEMFRGLYV VLEQFFRAPY TIYYPFEKGP VSPRFRGEHA LRRYPSGEER CIACKLCEAI CPALAITIDA EERIDGSRRT T KYDIDMTK CIYCGYCQES CPVDAIVETP NVEYATETRE ELLYNKEKLL ANGDKWELEL QYALDADAPY R

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Macromolecule #10: NUJM SUBUNIT

MacromoleculeName: NUJM SUBUNIT / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 20.849551 KDa
SequenceString: MSEHVHLPSP TFNTSSAGHF HPYNTLGLAT QSAMVGLGAG VVAAAARNSL ATGPRNILTT FSKSGGVVTI FTGASIAYVF TYCSAANLR ERKDGWNHMW AGAATGAVLG ARTKLVPAFI GWTVLCGAAC GLFGWTGARF NADRKASLEQ SPKGFVQEDA H QTFWEVVH ...String:
MSEHVHLPSP TFNTSSAGHF HPYNTLGLAT QSAMVGLGAG VVAAAARNSL ATGPRNILTT FSKSGGVVTI FTGASIAYVF TYCSAANLR ERKDGWNHMW AGAATGAVLG ARTKLVPAFI GWTVLCGAAC GLFGWTGARF NADRKASLEQ SPKGFVQEDA H QTFWEVVH RRPLSLTVEQ LGEGRGINAV PIATATEAPN

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Macromolecule #11: PSST SUBUNIT (NUKM)

MacromoleculeName: PSST SUBUNIT (NUKM) / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 23.455176 KDa
SequenceString: MLRSQIGRLA LRPTLVPATV IPQTRAYSAP AGTPRVSSSS MPTDFPLPSQ QKPNSAVDYT LTTLDAVANW ARQGSFWPVT FGLACCAVE MMHVSAPRYD QDRLGIIFRA SPRQSDIMIV AGTLTNKMAP VLRQVYDQMP EPRWVISMGS CANGGGYYHF S YSVVRGCD ...String:
MLRSQIGRLA LRPTLVPATV IPQTRAYSAP AGTPRVSSSS MPTDFPLPSQ QKPNSAVDYT LTTLDAVANW ARQGSFWPVT FGLACCAVE MMHVSAPRYD QDRLGIIFRA SPRQSDIMIV AGTLTNKMAP VLRQVYDQMP EPRWVISMGS CANGGGYYHF S YSVVRGCD RIVPVDVYVP GCPPTSEALM YGVFQLQRKM RNTKITRMWY RK

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Macromolecule #12: ND4L SUBUNIT (NULM)

MacromoleculeName: ND4L SUBUNIT (NULM) / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 9.482467 KDa
SequenceString:
MFIGTIILVL SFLGFVFNRR NIILAFICLE TMLLGINLIL LRNSVLFDDI SGSLFAIVII ILAGVESAIG LSLLVSYYRL RGVINS

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Macromolecule #13: NUMM SUBUNIT

MacromoleculeName: NUMM SUBUNIT / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 15.14897 KDa
SequenceString:
MLSRFVSKRA FSVSTQVSKS IISYNGNTIE IPEEYTKQAP NRDSTWAPAQ ASKTEIYKNN MVRFEQKDLS KQPMPYAGIE LIAQQPVRF VHGNTAVCDG ANHNGPGAQG HPKIFINVDA PGSHACQYCG TRYEKEH

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Macromolecule #14: ACPM1 SUBUNIT

MacromoleculeName: ACPM1 SUBUNIT / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 12.053585 KDa
SequenceString:
MLRNVSRLAL RSNFARQATM GQRFYSVARP DAEKRIAAVL ESFDKISNPA AITPTASFAK DLNLDSLDTV EVVVAIEEEF GIEIPDKEA DEIKSVNQAV EYILAQPDAK

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Macromolecule #15: NB4M SUBUNIT

MacromoleculeName: NB4M SUBUNIT / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 14.778818 KDa
SequenceString:
MAIIATAFAE TVKFSGSKQE LQKRTLALYR QFLRGAPTFA DLYEVQFSIP TIRTKIRQEF ERHRFVDDLS IQNVLYAKGH MEYQECINF WKQQAQFLKY FPEEDDIQGR HQPSNFVDKF LKNRA

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Macromolecule #16: ACPM2 SUBUNIT

MacromoleculeName: ACPM2 SUBUNIT / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 14.444458 KDa
SequenceString:
MLRQSVLRLS RAAVARPALS RSFVTAVARP QLVRAAPVSF IRHYSSAHVL TKDMIQERIV ALLESFDKVN DAKNITATAN LTSDLGLDS LDVVEVVMAI EEEFGLEIPD HDADEIKTVQ QAIDYVSAQP AAV

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Macromolecule #17: NI2M SUBUNIT

MacromoleculeName: NI2M SUBUNIT / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 12.90287 KDa
SequenceString:
MSHPVPFSAA NKKLVTSMYR QSLKLARNWI SNRQLFRQKA VEIRHKFDQN AQISNPRLLA RTLDETRAHL YEFRHPDPIV PPSFPGGTK YERNVPPRME KIMQHNLYEP

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Macromolecule #18: NESM SUBUNIT

MacromoleculeName: NESM SUBUNIT / type: protein_or_peptide / ID: 18 / Details: Polyalanine / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 13.549694 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #19: NUPM SUBUNIT

MacromoleculeName: NUPM SUBUNIT / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 19.355197 KDa
SequenceString:
MPREAAAHWV PFEDKANMPD NVPDVVEVGA TSAPLLSASY FIGAKCKPYN DDFMLCREES QGSGAIDCLK EGRRVTRCAV SVIEDINKS CLDEFRLHWQ CLEQNNHQLS GCRKAEALLN KCVFTKLNLE KKIPGLRPDE EPVFLKKDPW IKPAVDDFKS V RAYAEAKK NGTL

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Macromolecule #20: NB6M SUBUNIT

MacromoleculeName: NB6M SUBUNIT / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 14.112429 KDa
SequenceString:
MPSVGQDLPP VGGYEPVQWR RNLPARGFRP LVYLAALCGI CGYGFYRALG GIQERRELKR EKLWARIYLM PLLQAEEDRQ TVRRSIAQL EREKEIMKGT GFDVDKSVYN DGKFHAPALM IPPK

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Macromolecule #21: NUXM SUBUNIT

MacromoleculeName: NUXM SUBUNIT / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 18.588209 KDa
SequenceString:
MSSSTPLVKT SVNYSYGDYP LIDADPHFKR VVGYMRPSDY GVIGLATAAL PAGICFAEWL DPVKGKFARP SVKFLRVATM LGFAVGFGA AYARSSLRFF GVTENAREYK KDEAQMAARK AAGLEPYGTS SLTPELQEIA AKNSAHSIAG LFIFPWFNFV N HPYHGREQ K

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Macromolecule #22: NUYM SUBUNIT

MacromoleculeName: NUYM SUBUNIT / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 18.65608 KDa
SequenceString:
MLSRSLRQLS QPSVRSFATS ARLLQKKDVP EVGVNLDNVP AHEIVSGAPA ELSRNRVVRI YQQAKPATQS GEYGTFAWRL DWDIVDVAN RWENDLIGWQ SSGDYMQATQ MKFTSKESAI KFANKQGWDF YIQEPHHRKF RVKQYANNFV HSYGKLKHIR T K

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Macromolecule #23: NUZM SUBUNIT

MacromoleculeName: NUZM SUBUNIT / type: protein_or_peptide / ID: 23 / Details: Polyalanine / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 11.677386 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #24: NIAM SUBUNIT

MacromoleculeName: NIAM SUBUNIT / type: protein_or_peptide / ID: 24 / Details: Polyalanine / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 8.528504 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)

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Macromolecule #25: NEBM SUBUNIT

MacromoleculeName: NEBM SUBUNIT / type: protein_or_peptide / ID: 25 / Details: Polyalanine / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 5.464728 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #26: NB2M SUBUNIT

MacromoleculeName: NB2M SUBUNIT / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 6.948902 KDa
SequenceString:
MAPQLKDPWA RREAWRYQTN FTRANRFKGA APGFGIAVVA FGAYLAAEKF LFEKKDDHHH

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Macromolecule #27: NIDM SUBUNIT

MacromoleculeName: NIDM SUBUNIT / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 11.039276 KDa
SequenceString:
MSEHQRPELV SFDDINYNDH KKVREAQESY TREQFIRLEA LKTVRKALEK CYEESGPNHF EDCKNLAEQY LDMLPTHRLQ GYLGYQRND PSK

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Macromolecule #28: NUUM SUBUNIT

MacromoleculeName: NUUM SUBUNIT / type: protein_or_peptide / ID: 28 / Details: Polyalanine / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 3.847734 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #29: NI8M SUBUNIT

MacromoleculeName: NI8M SUBUNIT / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 9.621051 KDa
SequenceString:
MSAALREIRF HLCQNGSSSA PLRQFVKNQI GAFQKANPST KVLVREANGV KPIVFARFDH GHESKIGLDV SSEKEVAERV KSLIEAK

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Macromolecule #30: NI9M SUBUNIT

MacromoleculeName: NI9M SUBUNIT / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 7.233344 KDa
SequenceString:
MINANPGFWN GPFRYLRWSA HNRPHLFFAF AIGIAGPVAA LTLTPLRRKY LYPDHSPLPQ SYP

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Macromolecule #31: N7BM SUBUNIT

MacromoleculeName: N7BM SUBUNIT / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 16.175141 KDa
SequenceString:
MSSSLYRVLR NAWEVGPRSY WKQLNSIGDT KSGRLVGTDI YGNKFYETDH QDEIHLRTRY VEYKEKDYDM SQVEPGWHFW LGYGVDTAP CNTPKEKLPI RAYPYKFQPN YTGTPGAFVT YNTLKPKISA WEPVTKQRS

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Macromolecule #32: UNKNOWN SUBUNIT

MacromoleculeName: UNKNOWN SUBUNIT / type: protein_or_peptide / ID: 32 / Details: Unknown sequence Polyalanine / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 4.528573 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #33: NB5M SUBUNIT

MacromoleculeName: NB5M SUBUNIT / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 10.494885 KDa
SequenceString:
MVELKPSSAI QRGPLNKGGW DAPHALHNDG AIDRYAHWRT FYQERFKYTR ATGKSTLIFL VAFPALIGYV AYQSEGLFEF AGKRRGESV TTRG

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Macromolecule #34: NUNM SUBUNIT

MacromoleculeName: NUNM SUBUNIT / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 7.93277 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #35: ND1 SUBUNIT (NU1M)

MacromoleculeName: ND1 SUBUNIT (NU1M) / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 38.361266 KDa
SequenceString: MIINIVEILI FLVCVLFSVA YLTVAERKTL AYMQRRLGPN FVGYYGLLQA FADAVKLLLK EIVLPKESNY IILVISPLIT LITALIGWV VIPLGPGITL GELNLGILFS LAIGSLGVFG SLLSGWSSNS KYSLLGSIRS TAQLISYELI LTSIFIIIIM F VSSLNITT ...String:
MIINIVEILI FLVCVLFSVA YLTVAERKTL AYMQRRLGPN FVGYYGLLQA FADAVKLLLK EIVLPKESNY IILVISPLIT LITALIGWV VIPLGPGITL GELNLGILFS LAIGSLGVFG SLLSGWSSNS KYSLLGSIRS TAQLISYELI LTSIFIIIIM F VSSLNITT IIETQRVVWY CIPLLPLLLI FFIASVAETA RPPFDLTESE SELVAGYFTE YSGSPFVFFF LAEYSNIILI SA FNGYLLL GGYLSFNYSY LFNILFNDYS YVSFLFEGLI NSSAYAIKLV FLMFSFIWVR AAFPRFTYDN LINFCWIILL PLL FGIFLI IPSTLYIFDS FPTLI

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Macromolecule #36: ND2 SUBUNIT (NU2M)

MacromoleculeName: ND2 SUBUNIT (NU2M) / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 53.353359 KDa
SequenceString: MLILAIISLI TFVSMSKLSD NRAIIRLINI YLILVLVLDS FLYLLFLNNQ TYTVMGELLI FNSFTFYIDM LIYFIMIVIS SLYGYNLYN NNLYKTLFEP KKELIILFLI NILGALLIVH SNDFITLFVA IELQSYSIYL ITAIYNSSYK ASKASMLYFF M GGILSILI ...String:
MLILAIISLI TFVSMSKLSD NRAIIRLINI YLILVLVLDS FLYLLFLNNQ TYTVMGELLI FNSFTFYIDM LIYFIMIVIS SLYGYNLYN NNLYKTLFEP KKELIILFLI NILGALLIVH SNDFITLFVA IELQSYSIYL ITAIYNSSYK ASKASMLYFF M GGILSILI AYSINTYYSV LNSYTLHSLD SLIINTLDLN LILIALSLGL LFKIGIAPLH KWLISIYENT PILITIYISL IP KISILSY LVLSNISINS LVISILAILT LLVGSVGGLL QIKIKRLLAF SGLTNAGYMM LLLLLNNNEF SYLYYITQYS ISH LAIFMI IIFSIYYINY INNQYNPIIY VNQLKGLIHD NAYLVLSMAI VVFSFIGIPP LLGFFGKLNI LMSILNNGYY FISI VLIVA SLISALYYLY LLNVSIQDKN NILINSNETV SSVLSYILSS LIILITFGFI YNSLIIDIFN VYFN

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Macromolecule #37: ND3 SUBUNIT (NU3M)

MacromoleculeName: ND3 SUBUNIT (NU3M) / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 14.478329 KDa
SequenceString:
MNTFIIFIIL IPIVGFALLA VNILLAVYKP YNEKLGAFEC GLTSFNQTRL AFNAAFILVA ILFLPFDLEI STLLPYVMSI YLVSNYGFT IVLLFLLILI IGFVYEINTN ALKINKHNKP NTDSLIYKL

+
Macromolecule #38: ND4 SUBUNIT (NU4M)

MacromoleculeName: ND4 SUBUNIT (NU4M) / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 54.506641 KDa
SequenceString: MFLTSILLSS LYLFNRILAW QGNVKHFYLF ASNLLLLFIV VLYINFNTFS NSFQFNFELF NSLNPFGLSN SDISNGLLFG IDGLSLTFI LLTVLLIPLT LLGNWYNINF NSNLYYTLVL AIGLVILLNF WALDYISFYI LFEATLPLLF ILIHIYGSSD S ERASFYVL ...String:
MFLTSILLSS LYLFNRILAW QGNVKHFYLF ASNLLLLFIV VLYINFNTFS NSFQFNFELF NSLNPFGLSN SDISNGLLFG IDGLSLTFI LLTVLLIPLT LLGNWYNINF NSNLYYTLVL AIGLVILLNF WALDYISFYI LFEATLPLLF ILIHIYGSSD S ERASFYVL MFTLSGSLFM LLSIVVISIV LNTTNFINHN LFVLSLDLQT IIWLGLFIAI MVKTPLFPIH VWLPVVHSES PL AGSMILA GLILKLALYA ILRLLLPLLC EAQILYTPMI YIISLLTIIL TSLATLRQID LKVIIAYSSI SHMGIAILGV CSN TSLGIY GSIVLGVAHG FVSPALFLIV GGILYDRYHI RIVNYYKGLT TYMPQLATYI IILSFANIGT PLTGNFTGEF LSLQ GGFIR NPIIGGISCI SVLLAAIYQL KLTNKLTGGI SSIYMHRTND VTIREKFIMN ILIISTLIIG ICPQIMYNLL YWTVN NYIY II

+
Macromolecule #39: ND5 SUBUNIT (NU5M)

MacromoleculeName: ND5 SUBUNIT (NU5M) / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 73.740688 KDa
SequenceString: MYNAISLIII LPCISWLFPL FFGRQLGYVF VTRMTSTLII ITTLITYYYF YQLLGNNNPI NLELFNYLNI DYLDINYNFE IDALTITML LAITTISSMV HIYSIGYMET DPHQVRFFSL LSMFTFWMII LVTGSNYFVL FVGWEFIGVT SYLLISFWVT R LQAMKSAL ...String:
MYNAISLIII LPCISWLFPL FFGRQLGYVF VTRMTSTLII ITTLITYYYF YQLLGNNNPI NLELFNYLNI DYLDINYNFE IDALTITML LAITTISSMV HIYSIGYMET DPHQVRFFSL LSMFTFWMII LVTGSNYFVL FVGWEFIGVT SYLLISFWVT R LQAMKSAL SAVLMNRFGD AFFVLGLCVI AYVFGTLNYS TIFATAYLIN TDLLVLIMLA LFIAAMAKSA QFGLHNWLTL AM EGPTPVS SLLHAATLVT AGIYLLLRSA NILEYTPTVL FIILWIGALT TLSAGLIAIC SNDLKRIIAL STMSQLGMMT IAI GLSAYN LALFHLLGHA FFKALLFMSA GSIIHSILNE SQDIRTYGGL LSYLPYTYIC ITIASLSLMA MPGLTGYYTK DIII ESTYG SYSISNYVVY WIAYLSAVLT CVYSMKILYL TFYSNPNNNT ITYYNAHESN IYITLPMFIL AIFAMFAGWI LKDIY LGVG TDFVGTHILP NNFSYFDTEF SITQFYKLLP LISAILVSIL IVVLNEFFAI VFNLNNKYIN TVYSIFNQKL VSDQIL NHF IIFKGLVTSG NIAHHVDKGS LYRLGPVGIN RLLNKASYNV INLSSNTRSS LSMNSMLILI TIVSLLLLVL VMNVNFI IV IPVLISILYI LFS

+
Macromolecule #40: ND6 SUBUNIT (NU6M)

MacromoleculeName: ND6 SUBUNIT (NU6M) / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 20.765102 KDa
SequenceString:
MMYLTYYFIE ITIFLAILCT IFIISAKNPM VSILYMIALF VIAAMYLYLI GLGIFSLLYI MIYIGAIAVL FLFIITLLDI NSTELSVKS NIRDLPLVLI SLIVLTISGL MIYSNDSILI NKLLEAFGND YNTIITQDWF NIENTTLLTT IGNVLLTNNA F ILLVLAIV LLLGIIGPIS ITMKHKE

+
Macromolecule #41: NB8M SUBUNIT

MacromoleculeName: NB8M SUBUNIT / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 11.219027 KDa
SequenceString:
MAEFPPLLSQ EDMKKHKILL AYRDRCAALL VPLNECRKKN YYMPWACGHE RHEYEMCEVA DFQRRVKAMD KLKAEKIEQA KAAAAAAAA AAAADAEESK

+
Macromolecule #42: NIPM SUBUNIT

MacromoleculeName: NIPM SUBUNIT / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 10.035651 KDa
SequenceString:
MTAAGISLTG GRNRCFSEWQ SFMHCTAKTD AKSRAQCLPN FEDYMECLHH TKEKARLREI ESVLKQKKEG LEAPPVKVIP VKAIGLVEE

+
Macromolecule #43: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 43 / Number of copies: 6 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

+
Macromolecule #44: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 44 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

+
Macromolecule #45: FLAVIN MONONUCLEOTIDE

MacromoleculeName: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 45 / Number of copies: 1 / Formula: FMN
Molecular weightTheoretical: 456.344 Da
Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

+
Macromolecule #46: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

MacromoleculeName: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
type: ligand / ID: 46 / Number of copies: 1 / Formula: NDP
Molecular weightTheoretical: 745.421 Da
Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

+
Macromolecule #47: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 47 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #48: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

MacromoleculeName: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
type: ligand / ID: 48 / Number of copies: 1 / Formula: ZMP
Molecular weightTheoretical: 568.704 Da
Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

+
Macromolecule #49: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 49 / Number of copies: 1 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

+
Macromolecule #50: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE

MacromoleculeName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE / type: ligand / ID: 50 / Number of copies: 3 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration2 mg/mL
BufferpH: 7.2
Component:
ConcentrationFormulaName
20.0 mMTris
100.0 mMNaClSodium chloridesodium cloride
1.0 mMEDTAEthylenediaminetetraacetic acid
0.025 %DDM
GridModel: C-flat-1/1 / Material: COPPER / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 70 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI POLARA 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 45872 / Illumination mode: FLOOD BEAM / Imaging mode: OTHER / Cs: 2.0 mm / Nominal defocus max: -0.003 µm / Nominal defocus min: -0.0015 µm / Nominal magnification: 200000
Specialist opticsEnergy filter - Name: GIF Bioquantum
Sample stageCooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number grids imaged: 1 / Number real images: 3110 / Average exposure time: 8.0 sec. / Average electron dose: 60.5 e/Å2
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 271443
CTF correctionSoftware - Name: CTFFIND (ver. 4)
Startup modelType of model: EMDB MAP
EMDB ID:

4110

Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final 3D classificationNumber classes: 3 / Software - Name: RELION (ver. 2.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.32 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.1) / Number images used: 124626
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: OTHER
Output model

PDB-6gcs:
Cryo-EM structure of respiratory complex I from Yarrowia lipolytica

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