6GC8

50S ribosomal subunit assembly intermediate - 50S rec*

Summary for 6GC8

• Entry DOI: 10.2210/pdb6gc8/pdb
• EMDB information: 4378 4379 4380 4381 4382 4383
• Descriptor: 23S ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (32 entities in total)
• Functional Keywords: assembly precursor 48s 50s biogenesis in vitro reconstitution, ribosome
• Biological source: Escherichia coli K-12; More
• Total number of polymer chains: 29
• Total formula weight: 1323447.57

Authors

Nikolay, R.,Hilal, T.,Qin, B.,Loerke, J.,Buerger, J.,Mielke, T.,Spahn, C.M.T. (deposition date: 2018-04-17, release date: 2018-06-20, Last modification date: 2024-05-15)

Primary citation

Nikolay, R.,Hilal, T.,Qin, B.,Mielke, T.,Burger, J.,Loerke, J.,Textoris-Taube, K.,Nierhaus, K.H.,Spahn, C.M.T.
Structural Visualization of the Formation and Activation of the 50S Ribosomal Subunit during In Vitro Reconstitution.
Mol. Cell, 70:881-893.e3, 2018

PubMed Abstract: The assembly of ribosomal subunits is an essential prerequisite for protein biosynthesis in all domains of life. Although biochemical and biophysical approaches have advanced our understanding of ribosome assembly, our mechanistic comprehension of this process is still limited. Here, we perform an in vitro reconstitution of the Escherichia coli 50S ribosomal subunit. Late reconstitution products were subjected to high-resolution cryo-electron microscopy and multiparticle refinement analysis to reconstruct five distinct precursors of the 50S subunit with 4.3-3.8 Å resolution. These assembly intermediates define a progressive maturation pathway culminating in a late assembly particle, whose structure is more than 96% identical to a mature 50S subunit. Our structures monitor the formation and stabilization of structural elements in a nascent particle in unprecedented detail and identify the maturation of the rRNA-based peptidyl transferase center as the final critical step along the 50S assembly pathway.

PubMed: 29883607
DOI: 10.1016/j.molcel.2018.05.003

Experimental method

ELECTRON MICROSCOPY (3.8 Å)