6G5Y

The X-ray structure of the adduct formed in the reaction between lysozyme and a platinum(II) terpyridine compound (acid pH)

6G5Y の概要

• エントリーDOI: 10.2210/pdb6g5y/pdb
• 分子名称: Lysozyme C, NITRATE ION, PLATINUM (II) ION, ... (5 entities in total)
• 機能のキーワード: platinum terpyridine compounds, protein metalation, esi ms elucidation, x-ray structure determination, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16624.73

構造登録者

Merlino, A.,Ferraro, G. (登録日: 2018-03-30, 公開日: 2018-06-27, 最終更新日: 2024-11-13)

主引用文献

Ferraro, G.,Marzo, T.,Infrasca, T.,Cilibrizzi, A.,Vilar, R.,Messori, L.,Merlino, A.
A case of extensive protein platination: the reaction of lysozyme with a Pt(ii)-terpyridine complex.
Dalton Trans, 47:8716-8723, 2018

PubMed Abstract: An antiproliferative platinum(ii)-terpyridine complex bearing two piperidine substituents at positions 2 and 2' (compound 1, hereafter) interacts non-covalently with DNA and induces cell death through necrosis, i.e. a mode of action that is distinct from that exhibited by cisplatin (Suntharalingam, et al., Metallomics, 2013, 5, 514). Here, the interaction between this Pt compound and the model protein hen egg white lysozyme (HEWL) was studied by both electrospray ionization mass spectrometry (ESI MS) and X-ray crystallography. The ESI MS data collected after 24 h protein incubation with compound 1 at two different pH values offer evidence that the metal complex degrades upon reaction with HEWL, forming adducts with 1 : 1, 2 : 1 and 3 : 1 Pt/protein ratios. Two different X-ray structures of Pt-protein adducts, obtained by the reaction of HEWL with the Pt compound under different experimental conditions and incubation times, are then reported. An unexpected extensive platination of the protein is clearly observed: Pt containing fragments bind close to the NZ atom of Lys1 and OE1 atom of Glu7, NE2 atom of His15 and NH1 atom of Arg14, ND1 atom of His15, NZ atom of Lys96, NZ atom of Lys97 and ND1 atom of Asn93, NZ atom of Lys13 and the C-terminal carboxylate, and the N-terminal amine. An additional binding site was observed close to the NZ atom of Lys33. These results suggest that both N- and C-terminal tails, as well as Lys side chains, have to be considered as potential binding sites of Pt-containing drugs. The peculiar reactivity of compound 1 with biological macromolecules could play a role in its mode of action.

PubMed: 29904761
DOI: 10.1039/c8dt01254g

実験手法

X-RAY DIFFRACTION (1.49 Å)