Crystal structure of an engineered Botulinum Neurotoxin type B mutant E1191M/S1199Y in complex with human synaptotagmin 1

Summary for 6G5F

DescriptorBotulinum neurotoxin type B, Synaptotagmin-1, GLYCEROL, ... (5 entities in total)
Functional Keywordsbotulinum toxin, neurotoxin, protein engineering, receptor binding, toxin
Biological sourceClostridium botulinum
Total number of polymer chains3
Total molecular weight304983.75
Primary citation
Elliot, M.,Favre-Guilmard, C.,Liu, S.M.,Maignel, J.,Masuyer, G.,Beard, M.,Carre, D.,Kalinichev, M.,Lezmi, S.,Mir, I.,Nicoleau, C.,Palan, S.,Perier, C.,Raban, E.,Dong, M.,Stenmark, P.,Krupp, J.
Engineered botulinum neurotoxin B with improved binding to human receptors has enhanced efficacy in preclinical models
Sci Adv, 2019
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2311 0.2% 0.6% 1.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6g5f
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Molmil generated image of 6g5f
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Molmil generated image of 6g5f
rotated about y axis by 90°
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