6G5F
Crystal structure of an engineered Botulinum Neurotoxin type B mutant E1191M/S1199Y in complex with human synaptotagmin 1
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Botulinum neurotoxin type B | polymer | 1291 | 151066.8 | 2 | UniProt (P10844) Pfam (PF01742) Pfam (PF07952) Pfam (PF07953) Pfam (PF07951) In PDB | Clostridium botulinum | BoNT/B,Bontoxilysin-B |
2 | P | Synaptotagmin-1 | polymer | 21 | 2471.8 | 1 | UniProt (P21579) In PDB | Homo sapiens | Synaptotagmin I,SytI,p65 |
3 | A | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
4 | B | MALONATE ION | non-polymer | 102.0 | 1 | Chemie (MLI) | |||
5 | water | water | 18.0 | 268 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 1291 (UniProt: P10844)
PDB | External Database | Details |
---|---|---|
Gln 231 | Glu 231 | engineered mutation |
Tyr 234 | His 234 | engineered mutation |
Met 1191 | Glu 1191 | engineered mutation |
Tyr 1199 | Ser 1199 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 304605.4 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 378.3 | |
All* | Total formula weight | 304983.7 |