6G5F
Crystal structure of an engineered Botulinum Neurotoxin type B mutant E1191M/S1199Y in complex with human synaptotagmin 1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Botulinum neurotoxin type B | polymer | 1291 | 151066.8 | 2 | UniProt (P10844) Pfam (PF01742) Pfam (PF07952) Pfam (PF07953) Pfam (PF07951) | Clostridium botulinum | BoNT/B,Bontoxilysin-B |
| 2 | C (P) | Synaptotagmin-1 | polymer | 21 | 2471.8 | 1 | UniProt (P21579) | Homo sapiens | Synaptotagmin I,SytI,p65 |
| 3 | D, E, F (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 4 | G (B) | MALONATE ION | non-polymer | 102.0 | 1 | Chemie (MLI) | |||
| 5 | H, I (A, B) | water | water | 18.0 | 268 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 1291 (UniProt: P10844)
| PDB | External Database | Details |
|---|---|---|
| Gln 231 | Glu 231 | engineered mutation |
| Tyr 234 | His 234 | engineered mutation |
| Met 1191 | Glu 1191 | engineered mutation |
| Tyr 1199 | Ser 1199 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 304605.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 378.3 | |
| All* | Total formula weight | 304983.7 |
