6FZO

SMURFP-Y56F mutant

Summary for 6FZO

• Entry DOI: 10.2210/pdb6fzo/pdb
• Descriptor: Smurfp, SULFATE ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• Functional Keywords: smurfp, biliverdin, optoacustic, phycobiliprotein, fluorescent protein
• Biological source: synthetic construct
• Total number of polymer chains: 4
• Total formula weight: 64210.17

Authors

Janowski, R.,Fuenzalida-Wernera, J.P.,Mishra, K.,Vetschera, P.,Weidenfeld, I.,Richter, K.,Niessing, D.,Ntziachristos, V.,Stiel, A.C. (deposition date: 2018-03-15, release date: 2018-10-17, Last modification date: 2024-01-17)

Primary citation

Fuenzalida-Werner, J.P.,Janowski, R.,Mishra, K.,Weidenfeld, I.,Niessing, D.,Ntziachristos, V.,Stiel, A.C.
Crystal structure of a biliverdin-bound phycobiliprotein: Interdependence of oligomerization and chromophorylation.
J. Struct. Biol., 204:519-522, 2018

PubMed Abstract: Small, ultra-red fluorescence protein (smURFP) introduces the non-native biliverdin (BV) chromophore to phycobiliproteins (PBPs), allowing them to be used as transgenic labels for in vivo mammalian imaging. Presently, no structural information exists for PBPs bound to the non-native BV chromophore, which limits the further development of smURFP and related proteins as imaging labels or indicators. Here we describe the first crystal structure of a PBP bound to BV. The structures of smURFP-Y56R with BV and smURFP-Y56F without BV reveal unique oligomerization interfaces different from those in wild-type PBPs bound to native chromophores. Our structures suggest that the oligomerization interface affects the BV binding site, creating a link between oligomerization and chromophorylation that we confirmed through site-directed mutagenesis and that may help guide efforts to improve the notorious chromophorylation of smURFP and other PBPs engineered to bind BV.

PubMed: 30287387
DOI: 10.1016/j.jsb.2018.09.013

Experimental method

X-RAY DIFFRACTION (2.3 Å)