6FZO
SMURFP-Y56F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-08 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.310, 77.320, 79.490 |
| Unit cell angles | 90.00, 92.83, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.26344 |
| Rwork | 0.260 |
| R-free | 0.30860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4po5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.676 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.166 | 0.800 |
| Number of reflections | 21020 | |
| <I/σ(I)> | 10.94 | 1.99 |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 6.2 | 6.6 |
| CC(1/2) | 0.996 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 292 | 1.8 M ammonium sulphate, 0.1 M Bis-Tris buffer pH 5.25 |
