6FT6
Structure of the Nop53 pre-60S particle bound to the exosome nuclear cofactors
This is a non-PDB format compatible entry.
Summary for 6FT6
| Entry DOI | 10.2210/pdb6ft6/pdb |
| EMDB information | 4301 4302 |
| Descriptor | 7S ribosomal RNA, Bud site selection protein 20, 60S ribosomal protein L11-A, ... (60 entities in total) |
| Functional Keywords | rna exosome, ribosome, pre-ribosome, mtr4, helicase, rna |
| Biological source | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) More |
| Total number of polymer chains | 57 |
| Total formula weight | 2670685.70 |
| Authors | Schuller, J.M.,Falk, S.,Conti, E. (deposition date: 2018-02-20, release date: 2018-03-28, Last modification date: 2024-11-06) |
| Primary citation | Schuller, J.M.,Falk, S.,Fromm, L.,Hurt, E.,Conti, E. Structure of the nuclear exosome captured on a maturing preribosome. Science, 360:219-222, 2018 Cited by PubMed Abstract: The RNA exosome complex processes and degrades a wide range of transcripts, including ribosomal RNAs (rRNAs). We used cryo-electron microscopy to visualize the yeast nuclear exosome holocomplex captured on a precursor large ribosomal subunit (pre-60) during 7-to-5.8 rRNA processing. The cofactors of the nuclear exosome are sandwiched between the ribonuclease core complex (Exo-10) and the remodeled "foot" structure of the pre-60 particle, which harbors the 5.8 rRNA precursor. The exosome-associated helicase Mtr4 recognizes the preribosomal substrate by docking to specific sites on the 25 rRNA, captures the 3' extension of the 5.8 rRNA, and channels it toward Exo-10. The structure elucidates how the exosome forms a structural and functional unit together with its massive pre-60 substrate to process rRNA during ribosome maturation. PubMed: 29519915DOI: 10.1126/science.aar5428 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.9 Å) |
Structure validation
Download full validation report
