6FRM

Crystal Structure of coenzyme F420H2 oxidase (FprA) co-crystallized with 10 mM Tb-Xo4

Summary for 6FRM

• Entry DOI: 10.2210/pdb6frm/pdb
• Descriptor: Coenzyme F420H2 oxidase (FprA), FLAVIN MONONUCLEOTIDE, Tb-Xo4, ... (7 entities in total)
• Functional Keywords: tb-xo4, crystallophore, fpra, nucleation, phasing, anomalous, oxidoreductase
• Biological source: Methanothermococcus thermolithotrophicus
• Total number of polymer chains: 8
• Total formula weight: 377752.33

Authors

Engilberge, S.,Riobe, F.,Wagner, T.,Di Pietro, S.,Shima, S.,Girard, E.,Dumont, E.,Maury, O. (deposition date: 2018-02-16, release date: 2018-10-31, Last modification date: 2024-01-17)

Primary citation

Engilberge, S.,Riobe, F.,Wagner, T.,Di Pietro, S.,Breyton, C.,Franzetti, B.,Shima, S.,Girard, E.,Dumont, E.,Maury, O.
Unveiling the Binding Modes of the Crystallophore, a Terbium-based Nucleating and Phasing Molecular Agent for Protein Crystallography.
Chemistry, 24:9739-9746, 2018

PubMed Abstract: Crystallophores are lanthanide complexes that act as powerful auxiliary for protein crystallography due to their strong nucleating and phasing effects. To get first insights on the mechanisms behind nucleation induced by Crystallophore, we systematically identified various elaborated networks of supramolecular interactions between Tb-Xo4 and subset of 6 protein structures determined by X-ray diffraction in complex with terbium-Crystallophore (Tb-Xo4). Such interaction mapping analyses demonstrate the versatile binding behavior of the Crystallophore and pave the way to a better understanding of its unique properties.

PubMed: 29806881
DOI: 10.1002/chem.201802172

Experimental method

X-RAY DIFFRACTION (2.2 Å)