6FN2

X-ray structure of animal-like Cryptochrome from Chlamydomonas reinhardtii

6FN2 の概要

• エントリーDOI: 10.2210/pdb6fn2/pdb
• 分子名称: Cryptochrome photoreceptor, FLAVIN-ADENINE DINUCLEOTIDE, 8-HYDROXY-10-(D-RIBO-2,3,4,5-TETRAHYDROXYPENTYL)-5-DEAZAISOALLOXAZINE, ... (7 entities in total)
• 機能のキーワード: cryptochrome, photolyase, photoreceptor, flavoprotein
• 由来する生物種: Chlamydomonas reinhardtii (Chlamydomonas smithii)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 59362.64

構造登録者

Franz, S.,Ignatz, E.,Wenzel, S.,Zielosko, H.,Yamamoto, J.,Mittag, M.,Essen, L.-O. (登録日: 2018-02-02, 公開日: 2018-08-01, 最終更新日: 2024-05-08)

主引用文献

Franz, S.,Ignatz, E.,Wenzel, S.,Zielosko, H.,Putu, E.P.G.N.,Maestre-Reyna, M.,Tsai, M.D.,Yamamoto, J.,Mittag, M.,Essen, L.O.
Structure of the bifunctional cryptochrome aCRY from Chlamydomonas reinhardtii.
Nucleic Acids Res., 46:8010-8022, 2018

PubMed Abstract: Photolyases and cryptochromes form an almost ubiquitous family of blue light photoreceptors involved in the repair and maintenance of DNA integrity or regulatory control. We found that one cryptochrome from the green alga Chlamydomonas reinhardtii (CraCRY) is capable of both, control of transcript levels and the sexual cycle of the alga in a positive (germination) and negative manner (mating ability), as well as catalyzing the repair of UV-DNA lesions. Its 1.6 Å crystal structure shows besides the FAD chromophore an aromatic tetrad that is indispensable in animal-like type I cryptochromes for light-driven change of their signaling-active redox state and formation of a stable radical pair. Given CraCRY's catalytic activity as (6-4) photolyase in vivo and in vitro, we present the first co-crystal structure of a cryptochrome with duplex DNA comprising a (6-4) pyrimidine-pyrimidone lesion. This 2.9 Å structure reveals a distinct conformation for the catalytic histidine His1, H357, that challenges previous models of a single-photon driven (6-4) photolyase mechanism.

PubMed: 30032195
DOI: 10.1093/nar/gky621

実験手法

X-RAY DIFFRACTION (2.3 Å)