6FN2

X-ray structure of animal-like Cryptochrome from Chlamydomonas reinhardtii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.992
Spacegroup name	P 21 21 21
Unit cell lengths	150.249, 50.528, 64.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.800 - 2.300
R-factor	0.16963
Rwork	0.168
R-free	0.22753
RMSD bond length	0.009
RMSD bond angle	1.437
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.800	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.070
Number of reflections	22003
<I/σ(I)>	7.15
Completeness [%]	96.6
Redundancy	1.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M MES, pH 6.0 with 15 % PEG 5000