6FKW

Europium-containing methanol dehydrogenase

Summary for 6FKW

• Entry DOI: 10.2210/pdb6fkw/pdb
• Descriptor: Methanol dehydrogenase, EUROPIUM ION, PYRROLOQUINOLINE QUINONE, ... (4 entities in total)
• Functional Keywords: rare earth element, methanol dehydrogenase, pqq, oxidoreductase
• Biological source: Methylacidiphilum fumariolicum SolV
• Total number of polymer chains: 4
• Total formula weight: 256530.60

Authors

Barends, T.,Dietl, A. (deposition date: 2018-01-24, release date: 2018-03-21, Last modification date: 2024-11-13)

Primary citation

Jahn, B.,Pol, A.,Lumpe, H.,Barends, T.,Dietl, A.,Hogendoorn, C.,Op den Camp, H.,Daumann, L.
Similar but not the same: First Kinetic and Structural Analyses of a Methanol Dehydrogenase Containing a Europium Ion in the Active Site.
Chembiochem, 2018

PubMed Abstract: Since the discovery of the biological relevance of rare earth elements (REEs) for numerous different bacteria, questions concerning the advantages of REEs in the active sites of methanol dehydrogenases (MDHs) over calcium(II) and of why bacteria prefer light REEs have been a subject of debate. Here we report the cultivation and purification of the strictly REE-dependent methanotrophic bacterium Methylacidiphilum fumariolicum SolV with europium(III), as well as structural and kinetic analyses of the first methanol dehydrogenase incorporating Eu in the active site. Crystal structure determination of the Eu-MDH demonstrated that overall no major structural changes were induced by conversion to this REE. Circular dichroism (CD) measurements were used to determine optimal conditions for kinetic assays, whereas inductively coupled plasma mass spectrometry (ICP-MS) showed 70 % incorporation of Eu in the enzyme. Our studies explain why bacterial growth of SolV in the presence of Eu is significantly slower than in the presence of La /Ce /Pr : Eu-MDH possesses a decreased catalytic efficiency. Although REEs have similar properties, the differences in ionic radii and coordination numbers across the series significantly impact MDH efficiency.

PubMed: 29524328
DOI: 10.1002/cbic.201800130

Experimental method

X-RAY DIFFRACTION (1.4 Å)