6FIA
Structure of the human LINE-1 ORF1p coiled coil domain
Summary for 6FIA
Entry DOI | 10.2210/pdb6fia/pdb |
Related | 2w7a 2yko 2ykp 2ykq |
Descriptor | LINE-1 retrotransposable element ORF1 protein, CHLORIDE ION (3 entities in total) |
Functional Keywords | rna binding protein, coiled coil, genome evolution, nucleic acid chaperone, retrotransposition, rnp |
Biological source | Homo sapiens (Human) |
Cellular location | Nucleus, nucleolus : Q9UN81 |
Total number of polymer chains | 6 |
Total formula weight | 75725.23 |
Authors | Khazina, E.,Weichenrieder, O. (deposition date: 2018-01-17, release date: 2018-03-28, Last modification date: 2024-01-17) |
Primary citation | Khazina, E.,Weichenrieder, O. Human LINE-1 retrotransposition requires a metastable coiled coil and a positively charged N-terminus in L1ORF1p. Elife, 7:-, 2018 Cited by PubMed Abstract: LINE-1 (L1) is an autonomous retrotransposon, which acted throughout mammalian evolution and keeps contributing to human genotypic diversity, genetic disease and cancer. L1 encodes two essential proteins: L1ORF1p, a unique RNA-binding protein, and L1ORF2p, an endonuclease and reverse transcriptase. L1ORF1p contains an essential, but rapidly evolving N-terminal portion, homo-trimerizes via a coiled coil and packages L1RNA into large assemblies. Here, we determined crystal structures of the entire coiled coil domain of human L1ORF1p. We show that retrotransposition requires a non-ideal and metastable coiled coil structure, and a strongly basic L1ORF1p amino terminus. Human L1ORF1p therefore emerges as a highly calibrated molecular machine, sensitive to mutation but functional in different hosts. Our analysis rationalizes the locally rapid L1ORF1p sequence evolution and reveals striking mechanistic parallels to coiled coil-containing membrane fusion proteins. It also suggests how trimeric L1ORF1p could form larger meshworks and indicates critical novel steps in L1 retrotransposition. PubMed: 29565245DOI: 10.7554/eLife.34960 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.65 Å) |
Structure validation
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